2.3 Field Methods
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must be placed in two securely sealed plastic bags to prevent leakage.
The outer bag should be clearly marked “ice.” If using refrigerant, place
each refrigerant container in two securely sealed zipper-type plastic
bags to prevent sample bottle contamination in the event of leakage.
Place the refrigerant containers into insulated containers that will be
used for sample holding and transport. Provide enough refrigerant
to keep the samples cold until delivery to the lab or until placement
in a refrigerator if samples are to be stored at a staging area before
shipping.
• Transport the sample bottles and syringes, including the replicate
bottles and process blank bottles (if applicable), in the cooler that
will be used to store the samples. Bottles can be transferred to a small
cooler, suitable for carrying in a backpack, prior to departing from the
trailhead to access a site.
If desired, obtain one or more process blank bottles for transport to the
field, followed by return with samples to the lab. We consider this step to be
optional; many studies do not employ field blanks. Because the field blank
bottles are generally not opened in the field, laboratory blanks can be an
appropriate substitute that avoids the need for carrying more weight into
the field. *
2.3.1.3 Acquisition of Equipment, Supplies, and Data Forms
Each person or sample collection team should typically be provided the following equipment and materials:
• site information folders (including maps and Stream- or Lake-Water
Field-Data Forms)
• site documentation materials
• sampling protocol
• SOP documents for sampling and sample handling
• sampling bottles, and syringes if applicable
• each bottle preferably with label affixed, placed in a zipper-lock
plastic bag
• syringes placed in a lightweight plastic box with snap-on lid,
large enough to hold multiple syringes with plunger pulled threefourths of the way out
• plastic gloves stored in a secure plastic bag
• insulated containers, refrigerant, and backpacks
* Note that if sample filtering is to be performed in the field (not recommended in this protocol),
then field blanks should be transported to the field, filtered in the field, and returned to the
laboratory for analysis.
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