Conclusion and prospectives
78
This research aimed to investigate an optimized extraction approach for chitin and chitosan
from marine waste sources in Algeria, with the objectives of reducing time duration and reagent
consumption while achieving higher yields and characterization of the extracted chitin and
chitosan. Furthermore, the study aimed to explore the application potential of the resulting
chitosan in various fields, specifically in pharmaceuticals for wound healing and antiinflammatory purposes, as a bio stimulant for root propagation in agriculture, as films for food
preservation, and as a strength enhancer in the paper industry.
From the overall results obtained, it can be concluded that:
• The chitin extraction process resulted in a chitosan yield of 71% in this study. This
indicates a high efficiency in the conversion of chitin to chitosan, showcasing the
effectiveness of the extraction method employed.
• The research demonstrated that a diverse range of marine shell waste sources can be
utilized for chitin and chitosan extraction. This finding highlights the feasibility of using
various marine waste materials, thereby expanding the potential resources available for
sustainable chitosan production.
• Chitosan hydrochloride, a derivative of chitosan, exhibited superior properties
compared to chitosan itself. Chitosan hydrochloride has already been approved by
pharmacopeia and is widely used in the pharmaceutical industry. Its increased solubility
and enhanced antibacterial activity make it a promising candidate for pharmaceutical
applications.
• The antibacterial and antifungal potential of chitosan hydrochloride was investigated,
and positive results were observed. Chitosan hydrochloride displayed activity against
both gram-positive and gram-negative bacteria, as well as against fungal pathogens,
chitosan hydrochloride exhibited greater efficacy against gram-negative bacteria and
fungi, highlighting its broad-spectrum antimicrobial properties.
• The formulation of a chitosan hydrochloride-based cream for wound healing and antiinflammatory purposes proved successful. The cream formulation met pharmaceutical
standards and underwent testing in vivo on albino rabbits, demonstrating its potential
for therapeutic use. This outcome supports the development of chitosan-based
pharmaceutical products for wound healing and anti-inflammatory treatments.
• The experiment focusing on root enhancement using chitosan hydrochloride yielded
positive results. Chitosan hydrochloride demonstrated the ability to promote root growth
and enhance root propagation in agricultural applications. This finding suggests the
potential of chitosan hydrochloride as a biostimulant for plant cultivation, contributing
to sustainable agricultural practices.
78
This research aimed to investigate an optimized extraction approach for chitin and chitosan
from marine waste sources in Algeria, with the objectives of reducing time duration and reagent
consumption while achieving higher yields and characterization of the extracted chitin and
chitosan. Furthermore, the study aimed to explore the application potential of the resulting
chitosan in various fields, specifically in pharmaceuticals for wound healing and antiinflammatory purposes, as a bio stimulant for root propagation in agriculture, as films for food
preservation, and as a strength enhancer in the paper industry.
From the overall results obtained, it can be concluded that:
• The chitin extraction process resulted in a chitosan yield of 71% in this study. This
indicates a high efficiency in the conversion of chitin to chitosan, showcasing the
effectiveness of the extraction method employed.
• The research demonstrated that a diverse range of marine shell waste sources can be
utilized for chitin and chitosan extraction. This finding highlights the feasibility of using
various marine waste materials, thereby expanding the potential resources available for
sustainable chitosan production.
• Chitosan hydrochloride, a derivative of chitosan, exhibited superior properties
compared to chitosan itself. Chitosan hydrochloride has already been approved by
pharmacopeia and is widely used in the pharmaceutical industry. Its increased solubility
and enhanced antibacterial activity make it a promising candidate for pharmaceutical
applications.
• The antibacterial and antifungal potential of chitosan hydrochloride was investigated,
and positive results were observed. Chitosan hydrochloride displayed activity against
both gram-positive and gram-negative bacteria, as well as against fungal pathogens,
chitosan hydrochloride exhibited greater efficacy against gram-negative bacteria and
fungi, highlighting its broad-spectrum antimicrobial properties.
• The formulation of a chitosan hydrochloride-based cream for wound healing and antiinflammatory purposes proved successful. The cream formulation met pharmaceutical
standards and underwent testing in vivo on albino rabbits, demonstrating its potential
for therapeutic use. This outcome supports the development of chitosan-based
pharmaceutical products for wound healing and anti-inflammatory treatments.
• The experiment focusing on root enhancement using chitosan hydrochloride yielded
positive results. Chitosan hydrochloride demonstrated the ability to promote root growth
and enhance root propagation in agricultural applications. This finding suggests the
potential of chitosan hydrochloride as a biostimulant for plant cultivation, contributing
to sustainable agricultural practices.