Result and discussion
57
IV.
Optimization and Evaluation of Chitin and Chitosan Extraction Methods:
The primary objective of this experiment was to evaluate and optimize three distinct methods
for chitin and chitosan extraction (Table 13), the study aimed to determine the optimal
conditions for chitosan extraction by considering variations in acid and base concentrations,
temperature, duration, drying, and cleaning processes.
Table 13:Summary table of the 3 chitin and chitosan extraction method s
15g of the
powder
Method -01
Method - 02
Method- 03
ChitinYield
20%
33%
33%
Time
1h alkaline treatment
30 min acid treatment
1h alkalinetreatment
3h alkaline treatment
24h acetone
24h acidic treatment
12h alkaline treatment
48h alkaline treatment
24h acidic treatment
48h alkaline treatment
Heat
+
+
-
Reagent %
NAOH: 2%
HCL: 7%
NAOH: 50%
NAOH: 5%
HCL: 1%
NAOH: 55%
NAOH: 2%
HCL: 1%
NAOH: 48 %
Colour
Slight pink
White
Brown
Chitosan
amount (g)
1.5 g
3g
3g
Aspect
Viscosity