Materials and Methods
27
I.
Material
1. Biological Material
The biological material used in the present study is:
• Shrimp shell waste (10 kg)
• Pathogenic germs:
➢ Escherichia coli
➢ Staphylococcus aureus
➢ Pseudomonas aeruginosa
• Fungus
➢ Candida albicans
• Spider plants (Chlorophytum comosum)
• Commercial chitosan (RUIHERBS)
• Egg white
• Domesticated albino Rabbit (Oryctolagus cuniculus).
2. Non-Biological Material
The apparatus and reagents that were used in this study are listed in this table below:
Table 9: Apparatus and reagent used in this study
Apparatus
Reagent
• BALANCE
• BALLONS
• BAKERS
• BOROSILICATE FLASH
• BUNSEN BURNER
• CAPILARY RULER
• CENTRIFUGE
• CENTRIFUGE TUBES 50ML
• COFFEE GRINDER MACHINE
• COTTON DISKS
• COTTON SWABS
• DIGITAL ROTARY VISCOSIMETR
• DISSICATOR
• ERLENMEYER FLASK
• FILTRATION MEMBRANE
• FORCEPS
• FREEZER
• FT-IR SPECTRUM
• GLASS FILTRES
• GLASS FUNNEL
• GRADUATED TEST TUBES
• HEATED MAGNETIC STIRRER
• INCUBATOR
• INOCULATING LOOP
• MAGNETIC BARS
• ACETIC ACID
• ACETONE
• ALCHOOL BENZYLIC
• ALCHOOL CETO-STEARYLIC
• CETYLIQUE ALCHOOL
• STEARYL ALCHOOL
• AMMONIA
• AMMONIUM
• AMMONIUM THIOCYANATE
• BLEACH
• CETYL PALMITATE
• COOPER SULPHATE
• FERRIC SULPHATE
• GLYCERIN
• HCL
• ISOPROPYL MYRISTATE
• LIQUID PARAFFINE
• METHYL ORANGE
INDICATOR
• METHYL SALICYATE
• MONOSTEARATE
GLYCEROLE
• MONOSTEARATE
GLYCEROLE
• MUELLER HINTON AGAR