advances in molecular techniques and OMICs methods and point out some future
challenges.
1. Can these metagenomics approaches be powerful in tracking microbial diversity
and community dynamics at contaminated sites? If so, what are the essential goals
to enforce metagenomics methods to bioremediation?
2. Can proteomic techniques be used as a quantitative tool for tracking in situ
microbial metabolism? What are the technologically demanding situations in
making efficient use of such equipment in the field?
3. Can these microarrays be used as a excessive throughput, particular, sensitive,
and quantitative device for tracking microbial populations and activities?
4. Can OMICs tactics be used to clarify relationships among biomarker estimations
and in situ activities?
5. What is the level of correlation between the abundance and expression of the
practical genes and the rates of degradation or transformation of contaminants?
Can methods primarily based on OMICs offer insights into the costs of kinetic
predictions?
6. Can the collected data and information be scaled from molecules to subsurface
ecosystems for improving our predicative capability of field bioremediation?
10 Conclusions
Molecular biology approaches have shown an exponential rise in the past few
decades and, together with the latest developments in molecular nanotechnology,
will lead to further insight into bioremediation processes. Scientists are trying to
develop new bioremediation approaches by designing artificial pathways, developing databases for specific microbes or enzymes, and other inter- and intradisciplinary
metabolic pathways related to bioremediation. During this entire chapter, one common point is that in spite of the disadvantages of all the molecular-based approaches,
the idea that such cellular systems work has been clarified at some stage. In précis,
the OMICs tactics should be developed in a manner to assist finding solutions to
ecological questions in the context of biochemistry, physiology, microbiology,
geochemistry, and ecology.
References
Arora PK, Kumar M, Chauhan A, Raghava GP, Jain RK (2009) OxDBase: a database of
oxygenases involved in biodegradation. BMC Res Notes 2:67
Babalola OO, Kirby BM, Roes-Hill ML, Cook AE, Cary SC, Burton SG (2009) Phylogenetic
analysis of actinobacterial populations associated with Antarctic dry valley mineral soils.
Environ Microbiol 11:566–576
18 Molecular Technologies for Assessment of Bioremediation and. . .
465
challenges.
1. Can these metagenomics approaches be powerful in tracking microbial diversity
and community dynamics at contaminated sites? If so, what are the essential goals
to enforce metagenomics methods to bioremediation?
2. Can proteomic techniques be used as a quantitative tool for tracking in situ
microbial metabolism? What are the technologically demanding situations in
making efficient use of such equipment in the field?
3. Can these microarrays be used as a excessive throughput, particular, sensitive,
and quantitative device for tracking microbial populations and activities?
4. Can OMICs tactics be used to clarify relationships among biomarker estimations
and in situ activities?
5. What is the level of correlation between the abundance and expression of the
practical genes and the rates of degradation or transformation of contaminants?
Can methods primarily based on OMICs offer insights into the costs of kinetic
predictions?
6. Can the collected data and information be scaled from molecules to subsurface
ecosystems for improving our predicative capability of field bioremediation?
10 Conclusions
Molecular biology approaches have shown an exponential rise in the past few
decades and, together with the latest developments in molecular nanotechnology,
will lead to further insight into bioremediation processes. Scientists are trying to
develop new bioremediation approaches by designing artificial pathways, developing databases for specific microbes or enzymes, and other inter- and intradisciplinary
metabolic pathways related to bioremediation. During this entire chapter, one common point is that in spite of the disadvantages of all the molecular-based approaches,
the idea that such cellular systems work has been clarified at some stage. In précis,
the OMICs tactics should be developed in a manner to assist finding solutions to
ecological questions in the context of biochemistry, physiology, microbiology,
geochemistry, and ecology.
References
Arora PK, Kumar M, Chauhan A, Raghava GP, Jain RK (2009) OxDBase: a database of
oxygenases involved in biodegradation. BMC Res Notes 2:67
Babalola OO, Kirby BM, Roes-Hill ML, Cook AE, Cary SC, Burton SG (2009) Phylogenetic
analysis of actinobacterial populations associated with Antarctic dry valley mineral soils.
Environ Microbiol 11:566–576
18 Molecular Technologies for Assessment of Bioremediation and. . .
465