216
4 Notes
1. Avoid any contact of the glass container with the ultrasonic
probe to prevent any damage to glass beaker carrying
solution.
2. Wear hearing protectors before starting to use the device and
try to use a soundproof box to reduce sounds during process.
3. Before starting, make sure that you fix the transducer probe to
the booster horn and tighten it.
4. During sonication, transducer will generate heat; therefore
sensitive drugs should be continuously kept into an ice bath to
prevent excessive heating.
5. Circulation water bath used in this experiment is aimed to
minimize unstirring water layer effects.
SW480
ns
110
110
100
100
90
90
80
80
70
70
60
60
50
50
40
40
30
30
20
20
10
10
0
0
CNT
CNT
ETO
ETO
10 uM
10 uM
25 uM
25 uM
50 uM
50 uM
100 uM
100 uM
Ceranib Ceranib Ceranib Ceranib
Ceranib Ceranib Ceranib Ceranib
1:500
1:500
1:1000
1:1000
1:2000
1:2000
1:500
1:500
1:1000
1:1000
1:2000
1:2000
RE
RE
RE
RE
RE
RE
HELA
ns
ns
%VIABILITY
%VIABILITY
RE.Cer2
RE.Cer2
RE.Cer2
RE.Cer2
RE.Cer2
RE.Cer2
Fig. 10 The effect of ceranib-2 on cancer cells. SW480 and HeLa cells
Ali Ben Taleb et al.