7
6. The resulting solution has a 1:2 molar ratio of CA:2-HP-β-CD
(see Notes 4–6).
7. Immediately transfer this clear colorless solution at a freezer at
−80 °C (see Note 7).
8. Freeze-dry the resulting iced product to obtain a white fluffy
solid-state CA–2-HP-β-CD lyophilized product.
1. Accurately weigh 120 mg of RA and 964 mg of 2-HP-β-CD
and transfer in a 200 mL beaker.
2. Suspend with 100 mL of purified water.
3. Add small amounts of 5% v/v ammonium hydroxide solution
under magnetic stirring and pH monitoring (see Notes 1–3).
4. Keep pH at approximately 9-10.
5. After complete dissolution of the solids, fix the final volume at
120 mL with purified water.
6. Immediately transfer the resulting clear colorless solution (at
RA:2-HP-β-CD molar ratio of 1:2, see Notes 4–6) at a freezer
at −80 °C (see Note 7).
7. When it turns iced, freeze-dry to a lyophilizer to obtain the final
solid-state RA–2-HP-β-CD lyophilized product.
1. Accurately weigh 300 mg of SLB and 1860 mg of 2-HP-β-CD.
2. Transfer the weighed amounts of SLB and 2-HP-β-CD in a
glass vessel and suspend with 200 mL of purified water under
magnetic stirring.
3. Add small amounts of 5% v/v ammonium hydroxide solution
under continuous stirring and pH monitoring (pH should be
adjusted at approximately 10–10.5, see Notes 1–3).
4. After complete dissolution a slight pink clear solution should be
obtained.
5. Fix the final volume of the solution with purified water at
300 mL.
6. Freeze the resulting clear, pink solution (at a 1:2 SLB:2-HPβ-CD molar ratio, see Notes 4–6) at −80 °C (see Note 7).
7. Freeze-dry the iced product to obtain the solid-state inclusion
complex of SLB–2-HP-β-CD.
1. 150 mg of CRM and 1197 mg 2-HP-β-CD, accurately weighed,
should be mixed in a glass vessel containing 120 mL of purified
water (see Notes 4–6).
2. Then, add small amounts of 5% v/v ammonium hydroxide
solution to adjust the pH at approximately 10–10.5, under continuous stirring and pH monitoring (see Notes 1–3).
3.4.2 RA–2-HP-b-CD
Inclusion Complex
3.5 Steps to Follow
for the Preparation
of Solid State SLB–2HP- β-CD Inclusion
Complex [28]
3.6 Steps to Follow
for the Preparation
of Solid-State CRM–2HP- β-CD Inclusion
Complex
Neutralization and Freeze-Drying Technique to Prepare Drug: CD Complexes
6. The resulting solution has a 1:2 molar ratio of CA:2-HP-β-CD
(see Notes 4–6).
7. Immediately transfer this clear colorless solution at a freezer at
−80 °C (see Note 7).
8. Freeze-dry the resulting iced product to obtain a white fluffy
solid-state CA–2-HP-β-CD lyophilized product.
1. Accurately weigh 120 mg of RA and 964 mg of 2-HP-β-CD
and transfer in a 200 mL beaker.
2. Suspend with 100 mL of purified water.
3. Add small amounts of 5% v/v ammonium hydroxide solution
under magnetic stirring and pH monitoring (see Notes 1–3).
4. Keep pH at approximately 9-10.
5. After complete dissolution of the solids, fix the final volume at
120 mL with purified water.
6. Immediately transfer the resulting clear colorless solution (at
RA:2-HP-β-CD molar ratio of 1:2, see Notes 4–6) at a freezer
at −80 °C (see Note 7).
7. When it turns iced, freeze-dry to a lyophilizer to obtain the final
solid-state RA–2-HP-β-CD lyophilized product.
1. Accurately weigh 300 mg of SLB and 1860 mg of 2-HP-β-CD.
2. Transfer the weighed amounts of SLB and 2-HP-β-CD in a
glass vessel and suspend with 200 mL of purified water under
magnetic stirring.
3. Add small amounts of 5% v/v ammonium hydroxide solution
under continuous stirring and pH monitoring (pH should be
adjusted at approximately 10–10.5, see Notes 1–3).
4. After complete dissolution a slight pink clear solution should be
obtained.
5. Fix the final volume of the solution with purified water at
300 mL.
6. Freeze the resulting clear, pink solution (at a 1:2 SLB:2-HPβ-CD molar ratio, see Notes 4–6) at −80 °C (see Note 7).
7. Freeze-dry the iced product to obtain the solid-state inclusion
complex of SLB–2-HP-β-CD.
1. 150 mg of CRM and 1197 mg 2-HP-β-CD, accurately weighed,
should be mixed in a glass vessel containing 120 mL of purified
water (see Notes 4–6).
2. Then, add small amounts of 5% v/v ammonium hydroxide
solution to adjust the pH at approximately 10–10.5, under continuous stirring and pH monitoring (see Notes 1–3).
3.4.2 RA–2-HP-b-CD
Inclusion Complex
3.5 Steps to Follow
for the Preparation
of Solid State SLB–2HP- β-CD Inclusion
Complex [28]
3.6 Steps to Follow
for the Preparation
of Solid-State CRM–2HP- β-CD Inclusion
Complex
Neutralization and Freeze-Drying Technique to Prepare Drug: CD Complexes