145
1. Dissolve 10 mg of the triblocks in 4 mL of DMSO (see Note 15).
2. Add 5 mg of Dox (HCl salt) after obtaining a clear solution (in
the case of PEO-b-PBLG-b-PLL) or as lightly turbid solution
(in the case of PLL-b-PBLG-b-PLL).
3. Leave for half an hour to be dissolved.
4. Place the solution in a dialysis bag (see Note 16).
5. Dialyze against isotonic Tris buffer at pH = 7.4 (0.150 M NaCl,
0.010 M Tris) or isotonic carbonate buffer at pH = 10.5.
6. Take out the dialysis bag at pH = 10.5, to a 2 L solution of Tris
buffer at pH = 7.4 (see Note 17).
1. Dissolve 10 mg of polymer in 4 mL of DMSO (see Note 18).
2. Add 2 mg of Pacl, and leave for 1 h to be dissolved.
3. Place the solution in a dialysis bag (see Note 19).
3.5 Drug Loading
(See Fig. 3) [4, 5]
3.5.1 Doxorubicin
Loading
3.5.2 Pacl Loading
Fig. 3 Schematic representation of the vesicles formed with the encapsulated
drugs and the curvature of the interphase. The lower asymmetry in the hydrophilic blocks results in larger dimensions of the vesicles and lower curvature of
the interphase
Nanostructured Polymersomes for Controlled Drug Delivery