140
vesicles are not toxic. In vitro activity of the loaded vesicles against
human pancreatic cancer cell lines reveals comparable activity to
Myocet for the ABA loaded with doxorubicin, while lower activity
is observed for the ABC.
2 Materials
1. γ-Benzyl-l-glutamate (>99%).
2. l-Lysine (>99%).
3. Poly(ethylene oxide) end-functionalized monoamine used as
monofunctional macroinitiator.
4. N,N-dimethylformamide (DMF) (99.9+%, special grade for
peptide synthesis with less than 50 ppm of active impurities) is
the polymerization solvent (see Note 1).
5. 1,6-Diaminohexane (99.9%) serves as the initiator for the triblock copolypeptide (see Note 2).
6. Benzene (99%, thiophen free grade) (see Note 3).
7. Triphosgene (99%) was used as purchased.
Fig. 1 Reactions used for the synthesis of the amphiphilic triblock-co-polypeptides poly(l-lysine hydrochloride)-b-poly(γ-benzyl-d7-l-glutamate)-b-poly(l-lysine hydrochloride)
Hermis Iatrou et al.
vesicles are not toxic. In vitro activity of the loaded vesicles against
human pancreatic cancer cell lines reveals comparable activity to
Myocet for the ABA loaded with doxorubicin, while lower activity
is observed for the ABC.
2 Materials
1. γ-Benzyl-l-glutamate (>99%).
2. l-Lysine (>99%).
3. Poly(ethylene oxide) end-functionalized monoamine used as
monofunctional macroinitiator.
4. N,N-dimethylformamide (DMF) (99.9+%, special grade for
peptide synthesis with less than 50 ppm of active impurities) is
the polymerization solvent (see Note 1).
5. 1,6-Diaminohexane (99.9%) serves as the initiator for the triblock copolypeptide (see Note 2).
6. Benzene (99%, thiophen free grade) (see Note 3).
7. Triphosgene (99%) was used as purchased.
Fig. 1 Reactions used for the synthesis of the amphiphilic triblock-co-polypeptides poly(l-lysine hydrochloride)-b-poly(γ-benzyl-d7-l-glutamate)-b-poly(l-lysine hydrochloride)
Hermis Iatrou et al.