133
5. Finally, lyophilize the solution to obtain the final pentablock
terpolypeptide (Fig. 3) (see Note 9) as a white powder (see
Note 10).
1. Prepare pentablock terpolypeptide hydrogels very simply by
adding Milli-Q water in a vial containing the solid
polypeptide.
2. After 2–3 h, the hydrogel is ready without requiring any other
treatment. The water swells the polypeptide, which keeps the
general shape of the initial solid.
1. Prepare pentablock terpolypeptide hydrogels loaded with
gemcitabine (Hydrogem) by first dissolving the appropriate
amount of gemcitabine in pyrogen-free Milli-Q water
(gemcitabine:polypeptide ratio 1:1 (w/w)), at 35 °C, followed
by addition of the warm gemcitabine solution to the polypeptide. Keep the temperature at 35 °C until complete swelling of
the polypeptide and formation of homogeneous hydrogel,
about 3–4 h (see Note 11).
2. The hydrogel is stable at room temperature for several days,
while the same gemcitabine solution in Milli-Q water precipitates after 0.5 h when the temperature is lowered to 20 °C.
4 Notes
1. Dry triethylamine over calcium hydride for 1 day and then
distill and store in the vacuum line over sodium. The appropriate quantity needed is freshly distilled in a vacuum line.
3.4 Formation of
Hydrogels
3.5 Formation
of Hydrogels Loaded
with Gemcitabine [5, 6]
Fig. 2 Schematic illustration of pentablock terpolypeptide of the type PLys-b(PHIS-co-PBLG (or PLEU))-b-PLys-b-(PHIS-co-PBLG (or PLEU))-b-PLys
Nanostructured Hydrogels for Controlled Drug Delivery
5. Finally, lyophilize the solution to obtain the final pentablock
terpolypeptide (Fig. 3) (see Note 9) as a white powder (see
Note 10).
1. Prepare pentablock terpolypeptide hydrogels very simply by
adding Milli-Q water in a vial containing the solid
polypeptide.
2. After 2–3 h, the hydrogel is ready without requiring any other
treatment. The water swells the polypeptide, which keeps the
general shape of the initial solid.
1. Prepare pentablock terpolypeptide hydrogels loaded with
gemcitabine (Hydrogem) by first dissolving the appropriate
amount of gemcitabine in pyrogen-free Milli-Q water
(gemcitabine:polypeptide ratio 1:1 (w/w)), at 35 °C, followed
by addition of the warm gemcitabine solution to the polypeptide. Keep the temperature at 35 °C until complete swelling of
the polypeptide and formation of homogeneous hydrogel,
about 3–4 h (see Note 11).
2. The hydrogel is stable at room temperature for several days,
while the same gemcitabine solution in Milli-Q water precipitates after 0.5 h when the temperature is lowered to 20 °C.
4 Notes
1. Dry triethylamine over calcium hydride for 1 day and then
distill and store in the vacuum line over sodium. The appropriate quantity needed is freshly distilled in a vacuum line.
3.4 Formation of
Hydrogels
3.5 Formation
of Hydrogels Loaded
with Gemcitabine [5, 6]
Fig. 2 Schematic illustration of pentablock terpolypeptide of the type PLys-b(PHIS-co-PBLG (or PLEU))-b-PLys-b-(PHIS-co-PBLG (or PLEU))-b-PLys
Nanostructured Hydrogels for Controlled Drug Delivery