99
Thomas Mavromoustakos et al. (eds.), Supramolecules in Drug Discovery and Drug Delivery: Methods and Protocols,
Methods in Molecular Biology, vol. 2207, https://doi.org/10.1007/978-1-0716-0920-0_8,
© Springer Science+Business Media, LLC, part of Springer Nature 2021
Chapter 8
Drug Incorporation in the Drug Delivery System of Micelles
Evangelia Soumelidou, Simona Golič Grdadolnik,
and Thomas Mavromoustakos
Abstract
Micelles is a system frequently used for drug delivery. Drugs are incorporated and protected in micelles
before being delivered. Nuclear magnetic resonance is a suitable technique to detect the localization and
incorporation of drugs into the micelle system. Free radicals are used to further facilitate the probing of
the interactions between drug and micelles. This information is critical because drug-micelle interactions
determine how easily the drug will be released from micelles and therefore how easily will be delivered to
the target.
Key words Micelles, Captopril, NMR, 5-DSA spin label, Sodium dodecyl sulfate (SDS)
1 Introduction
It is well known that conventional drugs possess many shortcomings
in clinical applications such as inactivity in drug delivery, toxic
metabolization, and high hydrophobicity. Nanopharmaceutics has
been developed for optimizing the drug delivery to the target and
addressing the above mentioned shortcomings. Various vehicles are
well known to be developed to transfer the drugs selectively and
control their release. Micelles are among the loading systems used
especially for delivering drugs and controlling their release. In this
matrix-loading system, the drug is embedded in the nanocarrier
micelle and its release is determined by the degradation of the carrier
or diffusion [1]. In our study micelles are composed by the surfactant sodium dodecyl sulfate (SDS) which is an amphiphilic molecule
containing hydrophobic segment [CD 3 (CD 2 ) 11 ] and hydrophilic
SO 3
−
Na
+
. When its concentration exceeds the critical micelle concentration (CMC) it agglomerates in D 2 O because its solubility
becomes very low. The hydrophobic tails of SDS squeeze together
in the core in an attempt to minimize their contacts with water. The
hydrophilic heads from the other aspect are exposed to water in
order to decrease the system energy and provide stable forms [2].
Thomas Mavromoustakos et al. (eds.), Supramolecules in Drug Discovery and Drug Delivery: Methods and Protocols,
Methods in Molecular Biology, vol. 2207, https://doi.org/10.1007/978-1-0716-0920-0_8,
© Springer Science+Business Media, LLC, part of Springer Nature 2021
Chapter 8
Drug Incorporation in the Drug Delivery System of Micelles
Evangelia Soumelidou, Simona Golič Grdadolnik,
and Thomas Mavromoustakos
Abstract
Micelles is a system frequently used for drug delivery. Drugs are incorporated and protected in micelles
before being delivered. Nuclear magnetic resonance is a suitable technique to detect the localization and
incorporation of drugs into the micelle system. Free radicals are used to further facilitate the probing of
the interactions between drug and micelles. This information is critical because drug-micelle interactions
determine how easily the drug will be released from micelles and therefore how easily will be delivered to
the target.
Key words Micelles, Captopril, NMR, 5-DSA spin label, Sodium dodecyl sulfate (SDS)
1 Introduction
It is well known that conventional drugs possess many shortcomings
in clinical applications such as inactivity in drug delivery, toxic
metabolization, and high hydrophobicity. Nanopharmaceutics has
been developed for optimizing the drug delivery to the target and
addressing the above mentioned shortcomings. Various vehicles are
well known to be developed to transfer the drugs selectively and
control their release. Micelles are among the loading systems used
especially for delivering drugs and controlling their release. In this
matrix-loading system, the drug is embedded in the nanocarrier
micelle and its release is determined by the degradation of the carrier
or diffusion [1]. In our study micelles are composed by the surfactant sodium dodecyl sulfate (SDS) which is an amphiphilic molecule
containing hydrophobic segment [CD 3 (CD 2 ) 11 ] and hydrophilic
SO 3
−
Na
+
. When its concentration exceeds the critical micelle concentration (CMC) it agglomerates in D 2 O because its solubility
becomes very low. The hydrophobic tails of SDS squeeze together
in the core in an attempt to minimize their contacts with water. The
hydrophilic heads from the other aspect are exposed to water in
order to decrease the system energy and provide stable forms [2].