40
+
Recombinant vector containing soil
metagenomic DNA
E. coli cells
Function based analysis
Sequence based analysis
M icrobial Diversity
Complex soil microbial communities under diverse soil type and climate
Transformation in E. coli
Next Generation Sequencing
Microbial community structure
Library Screening
Metagenomic DNA library
High throughput Sequencing
Sequence Reads
Assembly of reads
Genome Sequence
Fig. 4.2 Key processes for metagenomics DNA library preparation to explore the soil microbial
community. In the sequential steps for development of the metagenomic DNA library from soil,
isolated soil DNA is fragmented and subsequently cloned in to the desired vector such as a plasmid, cosmid, fosmid, or BAC (bacterial artificial chromosome) followed by transformation into the
host cell. Further screening of desired clones with useful genes provides their ecophysiological
role in the associated ecosystem. Microbial community structure can be predicted directly from
soil metagenomic DNA via next-generation sequencing technologies
4 Single-Cell Genomics and Metagenomics for Microbial Diversity Analysis