Coarse-Grained Modeling and Simulations of Thermoresponsive …
57
Fig. 7 Melting curves for a series of neutral and charged CLP sequences. Ensemble average fraction
of intact glycine triplets (f intact ) as a function of reduced temperature (T * ) shows a the stabilizing
effect of increasing (POG) length, b the destabilizing effect of charged residues and c that altering
the balances of charged residues while holding sequence length constant does not significantly affect
the T m . d A legend showing the symbol and color scheme for each sequence. Parts of this figure
were adapted with permission from Ref. [106]. Copyright (2018) American Chemical Society
Table 1 Table of
computational melting
temperatures (T ∗
m ) for the
CLP sequences as shown in
Fig. 7
Sequence
Tm *
1
(PKG) 4 (POG) 4 (DOG) 4
4.38 ± 0.07
2
(PKG) 4 (POG) 6 (DOG) 4
4.46 ± 0.04
3
(POG) 12
5.10 ± 0.02
4
(POG) 14
5.17 ± 0.03
5
(PKG) 3 (POG) 7 (DOG) 4
4.53 ± 0.06
6
(PKG) 4 (POG) 7 (DOG) 3
4.57 ± 0.09
7
(POG) 6
4.63 ± 0.03
8
(POG) 7
4.74 ± 0.04
9
(POG) 8
4.85 ± 0.02
57
Fig. 7 Melting curves for a series of neutral and charged CLP sequences. Ensemble average fraction
of intact glycine triplets (f intact ) as a function of reduced temperature (T * ) shows a the stabilizing
effect of increasing (POG) length, b the destabilizing effect of charged residues and c that altering
the balances of charged residues while holding sequence length constant does not significantly affect
the T m . d A legend showing the symbol and color scheme for each sequence. Parts of this figure
were adapted with permission from Ref. [106]. Copyright (2018) American Chemical Society
Table 1 Table of
computational melting
temperatures (T ∗
m ) for the
CLP sequences as shown in
Fig. 7
Sequence
Tm *
1
(PKG) 4 (POG) 4 (DOG) 4
4.38 ± 0.07
2
(PKG) 4 (POG) 6 (DOG) 4
4.46 ± 0.04
3
(POG) 12
5.10 ± 0.02
4
(POG) 14
5.17 ± 0.03
5
(PKG) 3 (POG) 7 (DOG) 4
4.53 ± 0.06
6
(PKG) 4 (POG) 7 (DOG) 3
4.57 ± 0.09
7
(POG) 6
4.63 ± 0.03
8
(POG) 7
4.74 ± 0.04
9
(POG) 8
4.85 ± 0.02