6 Analytical Protocols in Chlorophyll Analysis
141
Table 6.4 Main characteristic product ions (m/z) of the chlorophylls present in phytoplankton
Chlorophyll
Product ions
Structural assignments
Chlorophyll c 1
[M+H-22] +
[M+H-Mg] +
Chlorophyll c 2
[M+H-60] +
Chlorophyll c 3
[M+H-18] +
[M+H-H 2 O] +
[8-ethyl]-chlorophyll c 3
[M+H-44] +
[M+H-CO 2 ] +
[M+H-77] +
[M+H-H 2 O-CO 2 CH 3 ] +
[8-vinyl]-phlide a
[M+H-22] +
[M+H-Mg] +
[7-methoxycarbonyl]-8-vinyl-phlide a [M+H-60] +
[M+H-42] +
[M+H-CH 2 CO] +
DV chlorophyll a
[M+H-278] +
[M+H-phytyl] +
[M+H-278-60] +
[M+H-phytyl-COCH 3 OH] +
[M+H-278-32] +
[M+H-phytyl-CH 3 OH] +
Chlorophyll d
[M+H-22] +
[M+H-Mg] +
[M+H-22-278] +
[M+H-Mg-phytyl] +
[M+H-22-278-60] + [M+H-Mg-phytyl-CO 2 CH 3 ] +
Chlorophyll f
[M+H-278] +
[M+H-phytyl] +
Bacteriochlorophyll c
Bacteriochlorophyll d
Bacteriochlorophyll e
[M+H-18] +
[M+H-H 2 O] +
[M+H-44] +
[M+H-CH 3 COH] +
[M+H-204] +
[M+H-farnesyl] +
[M+H-272] +
[M+H-geranylgeranyl] +
Bacteriochlorophyll a
[M+H-278] +
[M+H-phytyl] +
[M+H-60] +
[M+H-CH 3 HCO 2 ] +
[M+H-42] +
[M+H-CH 2 CO] +
Bacteriochlorophyll g
[M+H-22] +
[M+H-Mg] +
[M+H-204] +
[M+H-Mg-farnesyl] +
Chlorophyll a
Pheophytin a
[M+H-278] +
[M+H-phytyl] +
[M+H-46] +
[M+H-HCO 2 H] +
[M+H-60] +
[M+H-CH 3 HCO 2 ] +
Chlorophyll b
Pheophytin b
[M+H-278] +
[M+H-phytyl] +
[M+H-46] +
[M+H-HCO 2 H] +
[M+H-60] +
[M+H-CH 3 HCO 2 ] +
[M+H-28] +
[M+H-CO] +
Pyropheophytin a
[M+H-278] +
[M+H-phytyl] +
[M+H-46] +
[M+H-HCO 2 H] +
Chlorophyllide a
Chlorophyllide b
[M+H-60] +
[M+H-CH 3 HCO 2 ] +
Pheophorbide a
[M+H-32] +
[M+H-CH 3 OH] +
(continued)