7. Commercially available heating block/plate set at 40
C.
8. Plastic dishes (see Note 2).
9. Plastic transfer pipettes.
10. Hair knife: Make a thin end glass Pasteur pipet by pulling it on
a flame until it splits (it should look like a sharp pencil). Cut the
end and add some melted wax to glue a small piece of dark hair
(about 1 cm). Approach the hair to the flame very slightly to
make a ball shape. Be careful, it will burn very quickly.
11. High-precision grade tweezers numbers 5 and 3.
12. Stereoscope.
13. Upright confocal microscope with a 20Â or 40Â lens and a
motorized stage if multiple embryos are to be recorded.
Fig. 6 Tools and mould used to mount embryos for imaging. (a) Forceps, mould,
hair knife, and plastic transfer pipet. (b) mould mounted on a 60 mm petri dish
before addition of agarose. (c) Mould diagram. Dimensions in mm
Tools to Study Neural Crest Migration in Zebrafish
89
C.
8. Plastic dishes (see Note 2).
9. Plastic transfer pipettes.
10. Hair knife: Make a thin end glass Pasteur pipet by pulling it on
a flame until it splits (it should look like a sharp pencil). Cut the
end and add some melted wax to glue a small piece of dark hair
(about 1 cm). Approach the hair to the flame very slightly to
make a ball shape. Be careful, it will burn very quickly.
11. High-precision grade tweezers numbers 5 and 3.
12. Stereoscope.
13. Upright confocal microscope with a 20Â or 40Â lens and a
motorized stage if multiple embryos are to be recorded.
Fig. 6 Tools and mould used to mount embryos for imaging. (a) Forceps, mould,
hair knife, and plastic transfer pipet. (b) mould mounted on a 60 mm petri dish
before addition of agarose. (c) Mould diagram. Dimensions in mm
Tools to Study Neural Crest Migration in Zebrafish
89