Fig. 3 Image acquisition of the 3D spheroid invasion. (a(i) and a(ii)) Illustrations of the acquisition process. (a
(iii)) Representative maximum fluorescence intensity z-projected images of spheroid invasion 48 h after
embedding in ECM (taken with 10Â objective). White dashed lines depict some measurement of the lengths of
invasive strands (l 1 , l 2 and l 3 respectively). Cancer cells (red), CAFs (green) were mixed in 1:1 ratio. Scale bar:
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(iii)) Representative maximum fluorescence intensity z-projected images of spheroid invasion 48 h after
embedding in ECM (taken with 10Â objective). White dashed lines depict some measurement of the lengths of
invasive strands (l 1 , l 2 and l 3 respectively). Cancer cells (red), CAFs (green) were mixed in 1:1 ratio. Scale bar:
252
Sefora Conti et al.