1. ECM-coated substrate
2. Cell seeding
3. Matrigel addition
4. Cell growth
5. Analysis
DAPI/Podocalyxin/β-catenin/DIC
Fig. 3 Protocol to grow MDCK cells on ECM-coated flat micropatterns. Chips are
coated with a solution of purified ECM components (1), and cells are seeded on
top (2). Once cells adhere to the micropatterns, MG solution is added to medium
(3). Micropatterns are then placed in the incubator until cells reach a tubular
structure (4). At that point, they are suitable for cell imaging, or for biochemical
studies (5)
Micropatterned Tubes for Epithelial Analyses
235
2. Cell seeding
3. Matrigel addition
4. Cell growth
5. Analysis
DAPI/Podocalyxin/β-catenin/DIC
Fig. 3 Protocol to grow MDCK cells on ECM-coated flat micropatterns. Chips are
coated with a solution of purified ECM components (1), and cells are seeded on
top (2). Once cells adhere to the micropatterns, MG solution is added to medium
(3). Micropatterns are then placed in the incubator until cells reach a tubular
structure (4). At that point, they are suitable for cell imaging, or for biochemical
studies (5)
Micropatterned Tubes for Epithelial Analyses
235