2.2 Small Equipment
1. Dumont #5 forceps (Inox or Dumostar).
2. Small surgical scissors.
3. Tungsten needles and needle holder.
4. 35 mm plastic Petri dishes.
5. 35 mm Glass-bottom No. 1.5 dishes (MatTek) or micro-slides
of 15 conical wells with #1.5 tissue treated optically compatible
polymer bottom (for example, micro-angiogenesis slides Ibidi
Cat. No. 81506 or equivalent).
6. Embryo-tested lightweight mineral oil.
7. Glass pipettes or plastic transfer pipettes or P20 pipette for
embryo transfer (all have to be previously soaked in culture
medium to avoid embryos sticking to the pipette tip).
8. Hydroxytamoxifen suspended at 100 mg/mL in ethanol 100%,
and diluted in sesame oil to a final concentration of 10 mg/mL.
9. 1 mL Insulin syringe 25 g  16 mm.
2.3 Media
1. Dissection medium: Dulbecco’s modified Eagle medium
(DMEM)/F-12 supplemented with 10% fetal bovine serum
and 1% Penicillin-Streptomycin and L-glutamine and 15 mM
HEPES.
2. Culture Medium: Embryos in the peri-gastrulation (E5.5–
E7.75) period are cultured in 50% DMEM/F12 with L-glutamine and without phenol red for optimal imaging, 50% rat
Fig. 1 Microscope setup
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1. Dumont #5 forceps (Inox or Dumostar).
2. Small surgical scissors.
3. Tungsten needles and needle holder.
4. 35 mm plastic Petri dishes.
5. 35 mm Glass-bottom No. 1.5 dishes (MatTek) or micro-slides
of 15 conical wells with #1.5 tissue treated optically compatible
polymer bottom (for example, micro-angiogenesis slides Ibidi
Cat. No. 81506 or equivalent).
6. Embryo-tested lightweight mineral oil.
7. Glass pipettes or plastic transfer pipettes or P20 pipette for
embryo transfer (all have to be previously soaked in culture
medium to avoid embryos sticking to the pipette tip).
8. Hydroxytamoxifen suspended at 100 mg/mL in ethanol 100%,
and diluted in sesame oil to a final concentration of 10 mg/mL.
9. 1 mL Insulin syringe 25 g  16 mm.
2.3 Media
1. Dissection medium: Dulbecco’s modified Eagle medium
(DMEM)/F-12 supplemented with 10% fetal bovine serum
and 1% Penicillin-Streptomycin and L-glutamine and 15 mM
HEPES.
2. Culture Medium: Embryos in the peri-gastrulation (E5.5–
E7.75) period are cultured in 50% DMEM/F12 with L-glutamine and without phenol red for optimal imaging, 50% rat
Fig. 1 Microscope setup
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Wallis Nahaboo et al.