2018:450684.
https://doi.org/10.1101/
450684
15. Gilmour DT, Maischein H-M, Nu ¨sslein-Volhard C (2002) Migration and function of a
glial subtype in the vertebrate peripheral nervous system. Neuron 34:577–588. https://
doi.org/10.1016/S0896-6273(02)00683-9
16. Hochgreb-H€ agele T, Bronner ME (2013) A
novel FoxD3 gene trap line reveals neural
crest precursor movement and a role for
FoxD3 in their specification. Dev Biol
374:1–11. https://doi.org/10.1016/j.ydbio.
2012.11.035
17. Kaufman CK, Mosimann C, Fan ZP, Yang S,
Thomas AJ, Ablain J et al (2016) A zebrafish
melanoma model reveals emergence of neural
crest identity during melanoma initiation. Science 351:aad2197. https://doi.org/10.1126/
science.aad2197
18. Hoffman TL, Javier AL, Campeau SA, Knight
RD, Schilling TF (2007) Tfap2 transcription
factors in zebrafish neural crest development
and ectodermal evolution. J Exp Zoolog B
Mol Dev Evol 308B:679–691. https://doi.
org/10.1002/jez.b.21189
19. Dutton JR, Antonellis A, Carney TJ, Rodrigues
FS, Pavan WJ, Ward A et al (2008) An evolutionarily conserved intronic region controls the
spatiotemporal expression of the transcription
factor Sox10. BMC Dev Biol 8:105. https://
doi.org/10.1186/1471-213X-8-105
20. Das A, Crump JG (2012) Bmps and Id2a act
upstream of Twist1 to restrict Ectomesenchyme potential of the cranial neural crest. PLoS
Genet
8:e1002710.
https://doi.org/10.
1371/journal.pgen.1002710
21. Blasky AJ, Pan L, Moens CB, Appel B (2014)
Pard3 regulates contact between neural crest
cells and the timing of Schwann cell differentiation but is not essential for neural crest migration or myelination: PARD3 IN NC
MIGRATION AND SCHWANN CELL
MYELINATION. Dev Dyn 243:1511–1523.
https://doi.org/10.1002/dvdy.24172
22. Askary A, Mork L, Paul S, He X, Izuhara AK,
Gopalakrishnan S et al (2015) Iroquois proteins promote skeletal joint formation by maintaining chondrocytes in an immature state. Dev
Cell 35:358–365. https://doi.org/10.1016/j.
devcel.2015.10.004
23. Schilling TF, Pabic PL, Hoffman TL (2010)
Using transgenic zebrafish (Danio rerio) to
study development of the craniofacial skeleton.
J Appl Ichthyol 26:183–186. https://doi.org/
10.1111/j.1439-0426.2010.01401.x
24. Smith CJ, Morris AD, Welsh TG, Kucenas S
(2014) Contact-mediated inhibition between
oligodendrocyte progenitor cells and motor
exit point glia establishes the spinal cord transition zone. PLoS Biol 12:e1001961. https://
doi.org/10.1371/journal.pbio.1001961
25. Kucenas S, Takada N, Park H-C, Woodruff E,
Broadie K, Appel B (2008) CNS-derived glia
ensheath peripheral nerves and mediate motor
root development. Nat Neurosci 11:143–151.
https://doi.org/10.1038/nn2025
26. Dougherty M, Kamel G, Grimaldi M,
Gfrerer L, Shubinets V, Ethier R et al (2013)
Distinct requirements for wnt9a and irf6 in
extension and integration mechanisms during
zebrafish palate morphogenesis. Development
140:76–81.
https://doi.org/10.1242/dev.
080473
27. Prendergast A, Linbo TH, Swarts T, Ungos
JM, McGraw HF, Krispin S et al (2012) The
metalloproteinase inhibitor Reck is essential for
zebrafish DRG development. Development
139:1141–1152. https://doi.org/10.1242/
dev.072439
28. Balczerski B, Matsutani M, Castillo P,
Osborne N, Stainier DYR, Crump JG (2012)
Analysis of Sphingosine-1-phosphate signaling
mutants reveals endodermal requirements for
the growth but not dorsoventral patterning of
jaw skeletal precursors. Dev Biol 362:230–241.
https://doi.org/10.1016/j.ydbio.2011.12.010
29. Chung A-Y, Kim P-S, Kim S, Kim E, Kim D,
Jeong I et al (2013) Generation of demyelination models by targeted ablation of oligodendrocytes in the zebrafish CNS. Mol Cells
36:82–87. https://doi.org/10.1007/s10059013-0087-9
30. Rodrigues FSLM, Doughton G, Yang B, Kelsh
RN (2012) A novel transgenic line using the
Cre-lox system to allow permanent lineagelabeling of the zebrafish neural crest. Genesis
50:750–757. https://doi.org/10.1002/dvg.
22033
31. Mongera A, Singh AP, Levesque MP, Chen
Y-Y, Konstantinidis P, Nusslein-Volhard C
(2013) Genetic lineage labeling in zebrafish
uncovers novel neural crest contributions to
the head, including gill pillar cells. Development 140:916–925. https://doi.org/10.
1242/dev.091066
32. Scheer N, Riedl I, Warren JT, Kuwada JY,
Campos-Ortega JA (2002) A quantitative analysis of the kinetics of Gal4 activator and effector gene expression in the zebrafish. Mech Dev
112:9–14
106
Zain Alhashem et al.
https://doi.org/10.1101/
450684
15. Gilmour DT, Maischein H-M, Nu ¨sslein-Volhard C (2002) Migration and function of a
glial subtype in the vertebrate peripheral nervous system. Neuron 34:577–588. https://
doi.org/10.1016/S0896-6273(02)00683-9
16. Hochgreb-H€ agele T, Bronner ME (2013) A
novel FoxD3 gene trap line reveals neural
crest precursor movement and a role for
FoxD3 in their specification. Dev Biol
374:1–11. https://doi.org/10.1016/j.ydbio.
2012.11.035
17. Kaufman CK, Mosimann C, Fan ZP, Yang S,
Thomas AJ, Ablain J et al (2016) A zebrafish
melanoma model reveals emergence of neural
crest identity during melanoma initiation. Science 351:aad2197. https://doi.org/10.1126/
science.aad2197
18. Hoffman TL, Javier AL, Campeau SA, Knight
RD, Schilling TF (2007) Tfap2 transcription
factors in zebrafish neural crest development
and ectodermal evolution. J Exp Zoolog B
Mol Dev Evol 308B:679–691. https://doi.
org/10.1002/jez.b.21189
19. Dutton JR, Antonellis A, Carney TJ, Rodrigues
FS, Pavan WJ, Ward A et al (2008) An evolutionarily conserved intronic region controls the
spatiotemporal expression of the transcription
factor Sox10. BMC Dev Biol 8:105. https://
doi.org/10.1186/1471-213X-8-105
20. Das A, Crump JG (2012) Bmps and Id2a act
upstream of Twist1 to restrict Ectomesenchyme potential of the cranial neural crest. PLoS
Genet
8:e1002710.
https://doi.org/10.
1371/journal.pgen.1002710
21. Blasky AJ, Pan L, Moens CB, Appel B (2014)
Pard3 regulates contact between neural crest
cells and the timing of Schwann cell differentiation but is not essential for neural crest migration or myelination: PARD3 IN NC
MIGRATION AND SCHWANN CELL
MYELINATION. Dev Dyn 243:1511–1523.
https://doi.org/10.1002/dvdy.24172
22. Askary A, Mork L, Paul S, He X, Izuhara AK,
Gopalakrishnan S et al (2015) Iroquois proteins promote skeletal joint formation by maintaining chondrocytes in an immature state. Dev
Cell 35:358–365. https://doi.org/10.1016/j.
devcel.2015.10.004
23. Schilling TF, Pabic PL, Hoffman TL (2010)
Using transgenic zebrafish (Danio rerio) to
study development of the craniofacial skeleton.
J Appl Ichthyol 26:183–186. https://doi.org/
10.1111/j.1439-0426.2010.01401.x
24. Smith CJ, Morris AD, Welsh TG, Kucenas S
(2014) Contact-mediated inhibition between
oligodendrocyte progenitor cells and motor
exit point glia establishes the spinal cord transition zone. PLoS Biol 12:e1001961. https://
doi.org/10.1371/journal.pbio.1001961
25. Kucenas S, Takada N, Park H-C, Woodruff E,
Broadie K, Appel B (2008) CNS-derived glia
ensheath peripheral nerves and mediate motor
root development. Nat Neurosci 11:143–151.
https://doi.org/10.1038/nn2025
26. Dougherty M, Kamel G, Grimaldi M,
Gfrerer L, Shubinets V, Ethier R et al (2013)
Distinct requirements for wnt9a and irf6 in
extension and integration mechanisms during
zebrafish palate morphogenesis. Development
140:76–81.
https://doi.org/10.1242/dev.
080473
27. Prendergast A, Linbo TH, Swarts T, Ungos
JM, McGraw HF, Krispin S et al (2012) The
metalloproteinase inhibitor Reck is essential for
zebrafish DRG development. Development
139:1141–1152. https://doi.org/10.1242/
dev.072439
28. Balczerski B, Matsutani M, Castillo P,
Osborne N, Stainier DYR, Crump JG (2012)
Analysis of Sphingosine-1-phosphate signaling
mutants reveals endodermal requirements for
the growth but not dorsoventral patterning of
jaw skeletal precursors. Dev Biol 362:230–241.
https://doi.org/10.1016/j.ydbio.2011.12.010
29. Chung A-Y, Kim P-S, Kim S, Kim E, Kim D,
Jeong I et al (2013) Generation of demyelination models by targeted ablation of oligodendrocytes in the zebrafish CNS. Mol Cells
36:82–87. https://doi.org/10.1007/s10059013-0087-9
30. Rodrigues FSLM, Doughton G, Yang B, Kelsh
RN (2012) A novel transgenic line using the
Cre-lox system to allow permanent lineagelabeling of the zebrafish neural crest. Genesis
50:750–757. https://doi.org/10.1002/dvg.
22033
31. Mongera A, Singh AP, Levesque MP, Chen
Y-Y, Konstantinidis P, Nusslein-Volhard C
(2013) Genetic lineage labeling in zebrafish
uncovers novel neural crest contributions to
the head, including gill pillar cells. Development 140:916–925. https://doi.org/10.
1242/dev.091066
32. Scheer N, Riedl I, Warren JT, Kuwada JY,
Campos-Ortega JA (2002) A quantitative analysis of the kinetics of Gal4 activator and effector gene expression in the zebrafish. Mech Dev
112:9–14
106
Zain Alhashem et al.