a seed germinator for 8 days under 70% relative humidity at 25 Æ 2
C with 12 h
photoperiod following guidelines of ISTA [21] in three replicates with completely
randomized block design.
Seed germination and different growth characteristics such as radicle and plumule
length and vigor index were determined in control and treatment by the following
methods:
2.5 Germination Percentage
Total number of seeds germinated/total number of seeds taken for
germination  100.
2.6 Seedling Length
After 8 days of seed sowing, radicle and plumule length of the mung bean seedlings
was measured as per standard methods of ISTA [21]. The radicle and plumule length
was measured with a measuring scale, and values were expressed in centimeters.
2.7 Vigor Index
Vigor index of the mung bean seedlings was estimated according to the formula:
Vigor index ¼ Total seedling length mm
ð
ÞÂgermination percentage
[22].
2.8 Results and Discussion
In the present study, various parameters such as contact time, initial dye concentration, pH, adsorbent dose, and temperature were analyzed to study the effect of
activated carbon and fly ash on the adsorption of acid magenta dye.
Removal of Acid Magenta Dye by Fly Ash: A Sustainable Tool for Textile Effluent. . .
265
C with 12 h
photoperiod following guidelines of ISTA [21] in three replicates with completely
randomized block design.
Seed germination and different growth characteristics such as radicle and plumule
length and vigor index were determined in control and treatment by the following
methods:
2.5 Germination Percentage
Total number of seeds germinated/total number of seeds taken for
germination  100.
2.6 Seedling Length
After 8 days of seed sowing, radicle and plumule length of the mung bean seedlings
was measured as per standard methods of ISTA [21]. The radicle and plumule length
was measured with a measuring scale, and values were expressed in centimeters.
2.7 Vigor Index
Vigor index of the mung bean seedlings was estimated according to the formula:
Vigor index ¼ Total seedling length mm
ð
ÞÂgermination percentage
[22].
2.8 Results and Discussion
In the present study, various parameters such as contact time, initial dye concentration, pH, adsorbent dose, and temperature were analyzed to study the effect of
activated carbon and fly ash on the adsorption of acid magenta dye.
Removal of Acid Magenta Dye by Fly Ash: A Sustainable Tool for Textile Effluent. . .
265