can be used as a low-cost and efficient adsorbent for treatment of industrial effluents
due to its high porosity and large surface area. Therefore, present investigation was
conducted to compare the adsorption capacity of activated carbon and fly ash for
removal of acid magenta dye from aqueous solution and their impact on Vigna
radiata growth parameters.
2 Materials and Methods
The present investigation was conducted in the Plant Physiology Laboratory, Amity
Institute of Biotechnology, Amity University, Noida. Fly ash was collected from
Badarpur Thermal Power Station, Delhi.
2.1 Preparation of the Dye Stock Solution
Activated carbon and acid magenta dye were procured from Sigma Aldrich, India. It
is an anionic dye. The stock solution of acid magenta dye (1,000 ppm) was prepared
initially, and it was diluted appropriately to produce acid magenta dye solution of
desired concentrations. A calibration curve was plotted by analyzing different
concentrations of acid magenta dye solution by using UV-Visible double beam
spectrophotometer at 541 nm (Table 1).
Table 1 Details of acid magenta dye used in the experiment
Dyestuff
Acid fuchsin, acid violet 19
Appearance
Dark green
IUPAC name
Disodium 2-amino-5-[(Z)-(4-amino-3-sulfonatophenyl)(4-iminio-3-sulfonato2,5-cyclohexadien-1-ylidene)methyl]-3-methylbenzenesulfonate
Empirical
formula
C 20 H 17 N 3 Na 2 O 9 S 3
Molecular
weight
585.538 g/mol
Molecular
structure
λ max
541 nm
Removal of Acid Magenta Dye by Fly Ash: A Sustainable Tool for Textile Effluent. . .
263
due to its high porosity and large surface area. Therefore, present investigation was
conducted to compare the adsorption capacity of activated carbon and fly ash for
removal of acid magenta dye from aqueous solution and their impact on Vigna
radiata growth parameters.
2 Materials and Methods
The present investigation was conducted in the Plant Physiology Laboratory, Amity
Institute of Biotechnology, Amity University, Noida. Fly ash was collected from
Badarpur Thermal Power Station, Delhi.
2.1 Preparation of the Dye Stock Solution
Activated carbon and acid magenta dye were procured from Sigma Aldrich, India. It
is an anionic dye. The stock solution of acid magenta dye (1,000 ppm) was prepared
initially, and it was diluted appropriately to produce acid magenta dye solution of
desired concentrations. A calibration curve was plotted by analyzing different
concentrations of acid magenta dye solution by using UV-Visible double beam
spectrophotometer at 541 nm (Table 1).
Table 1 Details of acid magenta dye used in the experiment
Dyestuff
Acid fuchsin, acid violet 19
Appearance
Dark green
IUPAC name
Disodium 2-amino-5-[(Z)-(4-amino-3-sulfonatophenyl)(4-iminio-3-sulfonato2,5-cyclohexadien-1-ylidene)methyl]-3-methylbenzenesulfonate
Empirical
formula
C 20 H 17 N 3 Na 2 O 9 S 3
Molecular
weight
585.538 g/mol
Molecular
structure
λ max
541 nm
Removal of Acid Magenta Dye by Fly Ash: A Sustainable Tool for Textile Effluent. . .
263