Doctoral Studies in Biology and Biotechnology (POWR.03.05.0000-Z310/17), which is funded by the European Social Fund.
Part of this work was supported by “International scholarship
exchange of doctoral students and academic staff (PROM)”
financed by the polish National Agency for Academic Exchange
(NAWA). The project was implemented by University of Warmia
and Mazury in Olsztyn within the agreement with NAWA
no. PPI/PRO/2019/1/00024/U/00001. The work was done
by R. S. during the internship in Marine Research Institute in
Vigo, Spain, and for that this work was also supported by the
GAIN-Xunta de Galicia Project, Spain (IN607D 2017/01).
References
1. Carrera M, Can ˜as B, Gallardo JM (2012) Rapid
direct detection of the major fish allergen, parvalbumin, by selected MS/MS ion monitoring
mass spectrometry. J Proteome 75:3211–3220
2. Thompson A, Schafer J, Kuhn K, Kienle S,
Schwarz J, Schmidt G, Neumann T,
Johnstone R, Mohammed AK, Hamon C
(2003) Tandem mass tags: a novel quantification
strategy for comparative analysis of complex protein mixtures by MS/MS. Anal Chem
75:1895–1904
3. McAlister GC, Huttlin EL, Haas W, Ting L,
Jedrychowski MP, Rogers JC, Kuhn K, Pike I,
Grothe RA, Blethrow JD, Gygi SP (2012)
Increasing the multiplexing capacity of TMTs
using reporter ion isotopologues with isobaric
masses. Anal Chem 84(17):7469–7478
4. Rauniyar N, Yates JR (2014) Isobaric labelingbased relative quantification in shotgun proteomics. J Proteome Res 13:5293–5309
5. Stryin ´ ski R, Mateos J, Pascual S, Gonza ´lez AF,
Gallardo JM, Łopien ´ ska-Biernat E, Medina I,
Carrera M (2019) Proteome profiling of L3
and L4 Anisakis simplex development stages by
TMT-based quantitative proteomics. J Proteomics 201:1–11
6. Werner T, Becher I, Sweetman G, Doce C,
Savitski MM, Bantscheff M (2012) Highresolution enabled TMT 8-plexing. Anal Chem
84:7188–7194
7. Bai B, Tan H, Pagala VR, High AA, Ichhaporia
VP, Hendershot L, Peng J (2017) Deep
profiling of proteome and phosphoproteome
by isobaric labeling, extensive liquid chromatography, and mass spectrometry. In: Pyle AM,
Christianson DW (eds) Methods in enzymology,
1st edn. Elsevier, United Kingdom
8. Mattiucci S, Acerra V, Paoletti M, Cipriani P,
Levsen A, Webb SC, Canestrelli D, Nascetti G
(2016) No more time to stay ‘single’ in the
detection of Anisakis pegreffii, A. simplex (s.s.)
and hybridization events between them: a multimarker nuclear genotyping approach. Parasitology 143(8):998–1011
Shotgun Proteomics for Anisakis simplex
75
Part of this work was supported by “International scholarship
exchange of doctoral students and academic staff (PROM)”
financed by the polish National Agency for Academic Exchange
(NAWA). The project was implemented by University of Warmia
and Mazury in Olsztyn within the agreement with NAWA
no. PPI/PRO/2019/1/00024/U/00001. The work was done
by R. S. during the internship in Marine Research Institute in
Vigo, Spain, and for that this work was also supported by the
GAIN-Xunta de Galicia Project, Spain (IN607D 2017/01).
References
1. Carrera M, Can ˜as B, Gallardo JM (2012) Rapid
direct detection of the major fish allergen, parvalbumin, by selected MS/MS ion monitoring
mass spectrometry. J Proteome 75:3211–3220
2. Thompson A, Schafer J, Kuhn K, Kienle S,
Schwarz J, Schmidt G, Neumann T,
Johnstone R, Mohammed AK, Hamon C
(2003) Tandem mass tags: a novel quantification
strategy for comparative analysis of complex protein mixtures by MS/MS. Anal Chem
75:1895–1904
3. McAlister GC, Huttlin EL, Haas W, Ting L,
Jedrychowski MP, Rogers JC, Kuhn K, Pike I,
Grothe RA, Blethrow JD, Gygi SP (2012)
Increasing the multiplexing capacity of TMTs
using reporter ion isotopologues with isobaric
masses. Anal Chem 84(17):7469–7478
4. Rauniyar N, Yates JR (2014) Isobaric labelingbased relative quantification in shotgun proteomics. J Proteome Res 13:5293–5309
5. Stryin ´ ski R, Mateos J, Pascual S, Gonza ´lez AF,
Gallardo JM, Łopien ´ ska-Biernat E, Medina I,
Carrera M (2019) Proteome profiling of L3
and L4 Anisakis simplex development stages by
TMT-based quantitative proteomics. J Proteomics 201:1–11
6. Werner T, Becher I, Sweetman G, Doce C,
Savitski MM, Bantscheff M (2012) Highresolution enabled TMT 8-plexing. Anal Chem
84:7188–7194
7. Bai B, Tan H, Pagala VR, High AA, Ichhaporia
VP, Hendershot L, Peng J (2017) Deep
profiling of proteome and phosphoproteome
by isobaric labeling, extensive liquid chromatography, and mass spectrometry. In: Pyle AM,
Christianson DW (eds) Methods in enzymology,
1st edn. Elsevier, United Kingdom
8. Mattiucci S, Acerra V, Paoletti M, Cipriani P,
Levsen A, Webb SC, Canestrelli D, Nascetti G
(2016) No more time to stay ‘single’ in the
detection of Anisakis pegreffii, A. simplex (s.s.)
and hybridization events between them: a multimarker nuclear genotyping approach. Parasitology 143(8):998–1011
Shotgun Proteomics for Anisakis simplex
75