Preface
The present edition of Shotgun Proteomics: Methods and Protocols by Springer Nature is a
collection of specific shotgun proteomics-based laboratory techniques and applications
developed in leading laboratories and proteomics units worldwide.
In a shotgun proteomics approach, a mixture of proteins is digested with a protease, and
the resulting mixture of peptides is then subjected to single or multiple liquid chromatography separation and analyzed, mainly by mass spectrometry, using different fragmentation
approaches. Using bioinformatics and database searching algorithms, putative peptide
sequences are then validated and assigned to the identified proteins. This strategy has
revolutionized the discipline of proteomics. Currently, high-throughput shotgun proteomics approaches are powerful tools that achieve the identification and quantification of
thousands of proteins from a single analysis.
The book contains 20 chapters covering a broad range of topics divided in six main
parts: (I) shotgun proteomics of extracellular vesicles and subcellular structures (three
chapters), (II) shotgun proteomics in non-model organisms (three chapters), (III) clinical
proteomics (six chapters), (IV) food proteomics (two chapters), (V) analysis of posttranslational modifications and protein complexes (four chapters), and (VI) data processing
and storage (two chapters).
Chapters include introductions to their respective topics, lists of the necessary materials
and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
We are convinced that Shotgun Proteomics: Methods and Protocols will be an ideal and upto-date guide for researchers seeking to understand the proteome of any given biological
sample.
Vigo, Spain
M onica Carrera
Jesu ´ s Mateos
v
The present edition of Shotgun Proteomics: Methods and Protocols by Springer Nature is a
collection of specific shotgun proteomics-based laboratory techniques and applications
developed in leading laboratories and proteomics units worldwide.
In a shotgun proteomics approach, a mixture of proteins is digested with a protease, and
the resulting mixture of peptides is then subjected to single or multiple liquid chromatography separation and analyzed, mainly by mass spectrometry, using different fragmentation
approaches. Using bioinformatics and database searching algorithms, putative peptide
sequences are then validated and assigned to the identified proteins. This strategy has
revolutionized the discipline of proteomics. Currently, high-throughput shotgun proteomics approaches are powerful tools that achieve the identification and quantification of
thousands of proteins from a single analysis.
The book contains 20 chapters covering a broad range of topics divided in six main
parts: (I) shotgun proteomics of extracellular vesicles and subcellular structures (three
chapters), (II) shotgun proteomics in non-model organisms (three chapters), (III) clinical
proteomics (six chapters), (IV) food proteomics (two chapters), (V) analysis of posttranslational modifications and protein complexes (four chapters), and (VI) data processing
and storage (two chapters).
Chapters include introductions to their respective topics, lists of the necessary materials
and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
We are convinced that Shotgun Proteomics: Methods and Protocols will be an ideal and upto-date guide for researchers seeking to understand the proteome of any given biological
sample.
Vigo, Spain
M onica Carrera
Jesu ´ s Mateos
v