Acknowledgments
This work was supported by a 2014 CSUPERB New Investigator
Grant and the National Institute of General Medical Sciences of the
National Institutes of Health under Award Number
SC2GM111144 awarded to K.G.
References
1. Hanson JC, Tangrea MA, Kim S et al (2011)
Emmert-Buck, Expression microdissection
adapted to commercial laser dissection instruments. Nat Protoc 6:457–467
2. Gru ¨newald A, Rygiel KA, Hepplewhite PD
et al (2016) Mitochondrial DNA depletion in
respiratory chain-deficient Parkinson disease
neurons. Ann Neurol 79:366–378
3. Drummond ES, Nayak S, Ueberheide B, Wisniewski T (2015) Proteomic analysis of neurons microdissected from formalin-fixed,
paraffin-embedded Alzheimer’s disease brain
tissue. Sci Rep 5:15456
4. Nawandar DM, Wang A, Makielski K et al
(2015)
Differentiation-dependent
KLF4
expression promotes lytic Epstein-Barr virus
infection in epithelial cells. PLoS Pathog 11:
e1005195
5. Pflugradt R, Schmidt U, Landenberger B et al
(2011) A novel and effective separation
method for single mitochondria analysis. Mitochondrion 11:308–314
6. Zivraj KH, Tung YC, Piper M (2010) Subcellular profiling reveals distinct and developmentally regulated repertoire of growth cone
mRNAs. J Neurosci 30:15464–15478
7. Ezzoukhry Z, Henriet E, Cordelie `res FP
(2018) Combining laser capture microdissection and proteomics reveals an active translation machinery controlling invadosome
formation. Nat Commun 9:2031
8. Gutstein HB, Morris JS, Annangudi SP, Sweedler JV (2008) Microproteomics: analysis of
protein diversity in small samples. Mass Spectrom Rev 27:316–330
9. Gambade A, Zreika S, Gue ´guinou M et al
(2016) Activation of TRPV2 and BKCa channels by the LL-37 enantiomers stimulates calcium entry and migration of cancer cells.
Oncotarget.
https://doi.org/10.18632/
oncotarget.8122
10. Costanzo M, Abounit S, Marzo L et al (2013)
Transfer of polyglutamine aggregates in neuronal cells occurs in tunneling nanotubes. J Cell
Sci 126:3678–3685
11. Mo ¨ller J, Lu ¨hmann T, Chabria M et al (2013)
Macrophages lift off surface-bound bacteria
using a filopodium-lamellipodium hook-andshovel mechanism. Sci Rep 3:2884
12. Dent EW, Gupton SL, Gertler FB (2011) The
growth cone cytoskeleton in axon outgrowth
and guidance. Cold Spring Harb Perspect Biol
3:a001800
13. Van Audenhove I, Denert M, Boucherie C et al
(2016) Fascin rigidity and L-plastin flexibility
cooperate in cancer cell invadopodia and filopodia. J Biol Chem 291:9148–9160
14. Gousset K, Schiff E, Langevin C et al (2009)
Prions hijack tunnelling nanotubes for intercellular spread. Nat Cell Biol 11:328–336
15. Eugenin EA, Gaskill PJ, Berman JW (2009)
Tunneling nanotubes (TNT) are induced by
HIV-infection of macrophages: a potential
mechanism for intercellular HIV trafficking.
Cell Immunol 254:142–148
16. Thayanithy V, Dickson EL, Steer C et al (2014)
Tumor-stromal cross talk: direct cell-to-cell
transfer of oncogenic microRNAs via tunneling
nanotubes. Transl Res 164:359–365
17. Brayford S, Bryce NS, Schevzov G et al (2016)
Tropomyosin promotes lamellipodial persistence by collaborating with Arp2/3 at the leading edge. Curr Biol 26(10):1312–1318
18. Sherer NM, Mothes W (2008) Cytonemes and
tunneling nanotubules in cell-cell communication and viral pathogenesis. Trends Cell Biol
18:414–420
19. Thomsen R, Lade Nielsen A (2011) A Boyden
chamber-based method for characterization of
astrocyte protrusion localized RNA and protein. Glia 59:1782–1792
20. Kadiu I, Gendelman HE (2011) Human
immunodeficiency virus type 1 endocytic trafficking through macrophage bridging conduits
facilitates spread of infection. J Neuroimmune
Pharmacol 6:658–675
21. Mili S, Moissoglu K, Macara IG (2008)
Genome-wide screen identifies localized
RNAs anchored at cell protrusions through
microtubules and APC. Nature 453:115–119
44
Ana Gordon and Karine Gousset
This work was supported by a 2014 CSUPERB New Investigator
Grant and the National Institute of General Medical Sciences of the
National Institutes of Health under Award Number
SC2GM111144 awarded to K.G.
References
1. Hanson JC, Tangrea MA, Kim S et al (2011)
Emmert-Buck, Expression microdissection
adapted to commercial laser dissection instruments. Nat Protoc 6:457–467
2. Gru ¨newald A, Rygiel KA, Hepplewhite PD
et al (2016) Mitochondrial DNA depletion in
respiratory chain-deficient Parkinson disease
neurons. Ann Neurol 79:366–378
3. Drummond ES, Nayak S, Ueberheide B, Wisniewski T (2015) Proteomic analysis of neurons microdissected from formalin-fixed,
paraffin-embedded Alzheimer’s disease brain
tissue. Sci Rep 5:15456
4. Nawandar DM, Wang A, Makielski K et al
(2015)
Differentiation-dependent
KLF4
expression promotes lytic Epstein-Barr virus
infection in epithelial cells. PLoS Pathog 11:
e1005195
5. Pflugradt R, Schmidt U, Landenberger B et al
(2011) A novel and effective separation
method for single mitochondria analysis. Mitochondrion 11:308–314
6. Zivraj KH, Tung YC, Piper M (2010) Subcellular profiling reveals distinct and developmentally regulated repertoire of growth cone
mRNAs. J Neurosci 30:15464–15478
7. Ezzoukhry Z, Henriet E, Cordelie `res FP
(2018) Combining laser capture microdissection and proteomics reveals an active translation machinery controlling invadosome
formation. Nat Commun 9:2031
8. Gutstein HB, Morris JS, Annangudi SP, Sweedler JV (2008) Microproteomics: analysis of
protein diversity in small samples. Mass Spectrom Rev 27:316–330
9. Gambade A, Zreika S, Gue ´guinou M et al
(2016) Activation of TRPV2 and BKCa channels by the LL-37 enantiomers stimulates calcium entry and migration of cancer cells.
Oncotarget.
https://doi.org/10.18632/
oncotarget.8122
10. Costanzo M, Abounit S, Marzo L et al (2013)
Transfer of polyglutamine aggregates in neuronal cells occurs in tunneling nanotubes. J Cell
Sci 126:3678–3685
11. Mo ¨ller J, Lu ¨hmann T, Chabria M et al (2013)
Macrophages lift off surface-bound bacteria
using a filopodium-lamellipodium hook-andshovel mechanism. Sci Rep 3:2884
12. Dent EW, Gupton SL, Gertler FB (2011) The
growth cone cytoskeleton in axon outgrowth
and guidance. Cold Spring Harb Perspect Biol
3:a001800
13. Van Audenhove I, Denert M, Boucherie C et al
(2016) Fascin rigidity and L-plastin flexibility
cooperate in cancer cell invadopodia and filopodia. J Biol Chem 291:9148–9160
14. Gousset K, Schiff E, Langevin C et al (2009)
Prions hijack tunnelling nanotubes for intercellular spread. Nat Cell Biol 11:328–336
15. Eugenin EA, Gaskill PJ, Berman JW (2009)
Tunneling nanotubes (TNT) are induced by
HIV-infection of macrophages: a potential
mechanism for intercellular HIV trafficking.
Cell Immunol 254:142–148
16. Thayanithy V, Dickson EL, Steer C et al (2014)
Tumor-stromal cross talk: direct cell-to-cell
transfer of oncogenic microRNAs via tunneling
nanotubes. Transl Res 164:359–365
17. Brayford S, Bryce NS, Schevzov G et al (2016)
Tropomyosin promotes lamellipodial persistence by collaborating with Arp2/3 at the leading edge. Curr Biol 26(10):1312–1318
18. Sherer NM, Mothes W (2008) Cytonemes and
tunneling nanotubules in cell-cell communication and viral pathogenesis. Trends Cell Biol
18:414–420
19. Thomsen R, Lade Nielsen A (2011) A Boyden
chamber-based method for characterization of
astrocyte protrusion localized RNA and protein. Glia 59:1782–1792
20. Kadiu I, Gendelman HE (2011) Human
immunodeficiency virus type 1 endocytic trafficking through macrophage bridging conduits
facilitates spread of infection. J Neuroimmune
Pharmacol 6:658–675
21. Mili S, Moissoglu K, Macara IG (2008)
Genome-wide screen identifies localized
RNAs anchored at cell protrusions through
microtubules and APC. Nature 453:115–119
44
Ana Gordon and Karine Gousset