Consellerı ´a de Cultura, Educacio ´ n e Ordenacio ´ n Universitaria,
Xunta de Galicia (Centro Singular de investigacio ´ n de Galicia
accreditation 2019–2022, ED431G 2019/02), and the European
Regional Development Fund (ERDF) is also acknowledged.
References
1. Yuana Y, Sturk A, Nieuwland R (2013) Extracellular vesicles in physiological and pathological conditions. Blood Rev 27:31–39
2. Lo ¨tvall J, Hill AF, Hochberg F, Buza ´s EI, Di
Vizio D, Gardiner C, Gho YS, Kurochkin IV,
Mathivanan S, Quesenberry P, Susmita S,
Tahara H, Wauben MH, Witwer KW, The ´ry
C (2014) Minimal experimental requirements
for definition of extracellular vesicles and their
functions: a position statement from the International Society for Extracellular Vesicles. J
Extracell Vesicles 3:26913
3. Heijnen HF, Schiel AE, Fijnheer R, Geuze HJ,
Sixma JJ (1999) Activated platelets release two
types of membrane vesicles: microvesicles by
surface shedding and exosomes derived from
exocytosis of multivesicular bodies and alphagranules. Blood 94:3791–3799
4. Simons M, Raposo G (2009) Exosomes—
vesicular carriers for intercellular communication. Curr Opin Cell Biol 21:575–581
5. Colombo M, Moita C, van Niel G, Kowal J,
Vigneron J, Benaroch P, Manel N, Moita LF,
The ´ry C, Raposo G (2013) Analysis of ESCRT
functions in exosome biogenesis, composition
and secretion highlights the heterogeneity of
extracellular
vesicles.
J
Cell
Sci
126:5553–5565
6. Abels ER, Breakefield XO (2016) Introduction
to extracellular vesicles: biogenesis, RNA cargo
selection, content, release, and uptake. Cell
Mol Neurobiol 36:301–312
7. Gaceb A, Martinez MC, Andriantsitohaina R
(2014) Extracellular vesicles: new players in
cardiovascular diseases. Int J Biochem Cell
Biol 50:24–28
8. Ve ´lez P, Parguin ˜a AF, Ocaranza-Sa ´nchez R,
Grigorian-Shamagian L, Rosa I, AlonsoOrgaz S, de la Cuesta F, Guitia ´n E, Moreu J,
Barderas MG, Gonza ´lez-Juanatey JR, Garcı ´a A ´
(2014) Identification of a circulating microvesicle protein network involved in ST-elevation
myocardial infarction. Thromb Haemost
112:716–726
9. Barrachina MN, Sueiro AM, Casas V,
Izquierdo I, Hermida-Nogueira L, Guitia ´n E,
Casanueva FF, Abia ´n J, Carrascal M, Pardo M,
Garcı ´a A ´ (2019) A combination of proteomic
approaches identifies a panel of circulating
extracellular vesicle proteins related to the risk
of suffering cardiovascular disease in obese
patients. Proteomics 19:e1800248
10. Vila-Liante V, Sa ´nchez-Lo ´ pez V, Martı ´nezSales V, Ramo ´ n-Nun ˜ez LA, Arellano-Orden E,
Cano-Ruiz A, Rodrı ´guez-Martorell FJ, Gao L,
Otero-Candelera R (2016) Impact of sample
processing on the measurement of circulating
microparticles: storage and centrifugation
parameters.
Clin
Chem
Lab
Med
54:1759–1767
11. Ramacciotti E, Hawley AE, Wrobleski SK,
Myers DD, Strahler JR, Andrews PC, Guire
KE, Henke PK, Wakefield TW (2010) Proteomics of microparticles after deep venous
thrombosis. Thromb Res 125:e269–e274
12. Barrachina MN, Caldero ´n-Cruz B, FernandezRocca L, Garcı ´a A ´ (2019) Application of extracellular vesicles proteomics to cardiovascular
disease: guidelines, data analysis, and future
perspectives. Proteomics 19:e1800247
13. Shevchenko A, Jensen ON, Podtelejnikov AV,
Sagliocco F, Wilm M, Vorm O, Mortensen P,
Shevchenko A, Boucherie H, Mann M (1996)
Linking genome and proteome by mass spectrometry: large-scale identification of yeast proteins from two dimensional gels. Proc Natl
Acad Sci U S A 93:14440–14445
14. Shannon P, Markiel A, Ozier O, Baliga NS,
Wang
JT,
Ramage
D,
Amin
N,
Schwikowski B, Ideker T (2003) Cytoscape: a
software environment for integrated models of
biomolecular interaction networks. Genome
Res 13:2498–2504
15. Pathan M, Keerthikumar S, Chisanga D,
Alessandro R, Ang CS, Askenase P, Batagov
AO, Benito-Martin A, Camussi G, Clayton A,
Collino F, Di Vizio D, Farcon-Perez JM,
Fonseca P, Fonseka P, Fontana S, Gho YS,
Hendrix
A,
Hoen
EN,
Iraci
N,
Kastaniegaard K, Kislinger T, Kowal J, Kurochkin IV, Leonardi T, Liang Y, Llorente A, Lunavat TR, Maji S, Monteleone F, Øverbye A,
Panaretakis T, Patel T, Peinado H,
Pluchino S, Principe S, Ronquist G, Royo F,
Sahoo S, Spinelli C, Stensballe A, The ´ry C, van
Herwijnen MJC, Wauben M, Welton JL,
Zhao K, Mathivanan S (2017) A novel community driven software for functional enrichment analysis of extracellular vesicles data. J
Extracell Vesicles 6:1321455
Circulating EVs Clinical Proteomics
23
Xunta de Galicia (Centro Singular de investigacio ´ n de Galicia
accreditation 2019–2022, ED431G 2019/02), and the European
Regional Development Fund (ERDF) is also acknowledged.
References
1. Yuana Y, Sturk A, Nieuwland R (2013) Extracellular vesicles in physiological and pathological conditions. Blood Rev 27:31–39
2. Lo ¨tvall J, Hill AF, Hochberg F, Buza ´s EI, Di
Vizio D, Gardiner C, Gho YS, Kurochkin IV,
Mathivanan S, Quesenberry P, Susmita S,
Tahara H, Wauben MH, Witwer KW, The ´ry
C (2014) Minimal experimental requirements
for definition of extracellular vesicles and their
functions: a position statement from the International Society for Extracellular Vesicles. J
Extracell Vesicles 3:26913
3. Heijnen HF, Schiel AE, Fijnheer R, Geuze HJ,
Sixma JJ (1999) Activated platelets release two
types of membrane vesicles: microvesicles by
surface shedding and exosomes derived from
exocytosis of multivesicular bodies and alphagranules. Blood 94:3791–3799
4. Simons M, Raposo G (2009) Exosomes—
vesicular carriers for intercellular communication. Curr Opin Cell Biol 21:575–581
5. Colombo M, Moita C, van Niel G, Kowal J,
Vigneron J, Benaroch P, Manel N, Moita LF,
The ´ry C, Raposo G (2013) Analysis of ESCRT
functions in exosome biogenesis, composition
and secretion highlights the heterogeneity of
extracellular
vesicles.
J
Cell
Sci
126:5553–5565
6. Abels ER, Breakefield XO (2016) Introduction
to extracellular vesicles: biogenesis, RNA cargo
selection, content, release, and uptake. Cell
Mol Neurobiol 36:301–312
7. Gaceb A, Martinez MC, Andriantsitohaina R
(2014) Extracellular vesicles: new players in
cardiovascular diseases. Int J Biochem Cell
Biol 50:24–28
8. Ve ´lez P, Parguin ˜a AF, Ocaranza-Sa ´nchez R,
Grigorian-Shamagian L, Rosa I, AlonsoOrgaz S, de la Cuesta F, Guitia ´n E, Moreu J,
Barderas MG, Gonza ´lez-Juanatey JR, Garcı ´a A ´
(2014) Identification of a circulating microvesicle protein network involved in ST-elevation
myocardial infarction. Thromb Haemost
112:716–726
9. Barrachina MN, Sueiro AM, Casas V,
Izquierdo I, Hermida-Nogueira L, Guitia ´n E,
Casanueva FF, Abia ´n J, Carrascal M, Pardo M,
Garcı ´a A ´ (2019) A combination of proteomic
approaches identifies a panel of circulating
extracellular vesicle proteins related to the risk
of suffering cardiovascular disease in obese
patients. Proteomics 19:e1800248
10. Vila-Liante V, Sa ´nchez-Lo ´ pez V, Martı ´nezSales V, Ramo ´ n-Nun ˜ez LA, Arellano-Orden E,
Cano-Ruiz A, Rodrı ´guez-Martorell FJ, Gao L,
Otero-Candelera R (2016) Impact of sample
processing on the measurement of circulating
microparticles: storage and centrifugation
parameters.
Clin
Chem
Lab
Med
54:1759–1767
11. Ramacciotti E, Hawley AE, Wrobleski SK,
Myers DD, Strahler JR, Andrews PC, Guire
KE, Henke PK, Wakefield TW (2010) Proteomics of microparticles after deep venous
thrombosis. Thromb Res 125:e269–e274
12. Barrachina MN, Caldero ´n-Cruz B, FernandezRocca L, Garcı ´a A ´ (2019) Application of extracellular vesicles proteomics to cardiovascular
disease: guidelines, data analysis, and future
perspectives. Proteomics 19:e1800247
13. Shevchenko A, Jensen ON, Podtelejnikov AV,
Sagliocco F, Wilm M, Vorm O, Mortensen P,
Shevchenko A, Boucherie H, Mann M (1996)
Linking genome and proteome by mass spectrometry: large-scale identification of yeast proteins from two dimensional gels. Proc Natl
Acad Sci U S A 93:14440–14445
14. Shannon P, Markiel A, Ozier O, Baliga NS,
Wang
JT,
Ramage
D,
Amin
N,
Schwikowski B, Ideker T (2003) Cytoscape: a
software environment for integrated models of
biomolecular interaction networks. Genome
Res 13:2498–2504
15. Pathan M, Keerthikumar S, Chisanga D,
Alessandro R, Ang CS, Askenase P, Batagov
AO, Benito-Martin A, Camussi G, Clayton A,
Collino F, Di Vizio D, Farcon-Perez JM,
Fonseca P, Fonseka P, Fontana S, Gho YS,
Hendrix
A,
Hoen
EN,
Iraci
N,
Kastaniegaard K, Kislinger T, Kowal J, Kurochkin IV, Leonardi T, Liang Y, Llorente A, Lunavat TR, Maji S, Monteleone F, Øverbye A,
Panaretakis T, Patel T, Peinado H,
Pluchino S, Principe S, Ronquist G, Royo F,
Sahoo S, Spinelli C, Stensballe A, The ´ry C, van
Herwijnen MJC, Wauben M, Welton JL,
Zhao K, Mathivanan S (2017) A novel community driven software for functional enrichment analysis of extracellular vesicles data. J
Extracell Vesicles 6:1321455
Circulating EVs Clinical Proteomics
23