bioactive collagen peptides, antihypertensive, antimicrobial, and
antitumor peptides were predicted as potential valuable bioactive
peptides from seafood and marine by-products. Thus, the combination of the global shotgun proteomic analysis with their analysis
by protein-based bioinformatics shows a rapid strategy for the
characterization of new potential food bioactive peptides. All
these potential bioactive peptides need to be validated by further
bioactivity assays using synthetic versions of the peptides. Nevertheless, compared with the classical approaches, the bioinformatic
methods are faster and lower-cost alternatives that predict and
reduce the number of potential targets to be investigated.
2 Materials
2.1 Protein
Extraction
1. Lysis buffer: 10 mM Tris–HCl, pH 7.2 with 5 mM phenylmethylsulfonyl fluoride (PMSF). Prepared at the moment and
stored at 4
C.
2. Ultra-Turrax device (IKA-Werke, Staufen, Germany).
3. Centrifuge.
Fig. 1 Workflow for the characterization of food-derived bioactive peptides presented in this chapter
Shotgun Proteomics and Protein-ased Bioinformatics. . .
217
antitumor peptides were predicted as potential valuable bioactive
peptides from seafood and marine by-products. Thus, the combination of the global shotgun proteomic analysis with their analysis
by protein-based bioinformatics shows a rapid strategy for the
characterization of new potential food bioactive peptides. All
these potential bioactive peptides need to be validated by further
bioactivity assays using synthetic versions of the peptides. Nevertheless, compared with the classical approaches, the bioinformatic
methods are faster and lower-cost alternatives that predict and
reduce the number of potential targets to be investigated.
2 Materials
2.1 Protein
Extraction
1. Lysis buffer: 10 mM Tris–HCl, pH 7.2 with 5 mM phenylmethylsulfonyl fluoride (PMSF). Prepared at the moment and
stored at 4
C.
2. Ultra-Turrax device (IKA-Werke, Staufen, Germany).
3. Centrifuge.
Fig. 1 Workflow for the characterization of food-derived bioactive peptides presented in this chapter
Shotgun Proteomics and Protein-ased Bioinformatics. . .
217