Urine Samples
1. Microfuge.
2. Eppendorf clear.
3. Amicon
® Ultra 3K device.
Blood, Serum, or Plasma
Samples
1. Microfuge.
2. Eppendorf clear.
3. Blood
collection
tubes
containing
K2-ethylenediaminetetraacetic acid (EDTA).
4. Blood collection tubes without any additives.
5. Methanol (MeOH).
6. Chloroform (CHCl 3 ).
2.2 Major Protein
Removal
2.2.1 Dithiothreitol (DTT)
1. 500 mM DTT in Milli-Q water; store at À20
C.
2. Microfuge.
3. Vortex mixer.
4. Eppendorf clear.
2.2.2 Nanoparticles for
Protein Enrichment
Nanoparticles in Blood
Serum Plasma Samples
Au, Ag, and Pt
Nanoparticles
1. Gold nanoparticles (AuNPs).
2. Silver nanoparticles (AgNPs).
3. Platinum nanoparticles (PtNPs).
4. Miller-GP
® Filter Unit with a size of 0.22 μm.
5. 500 mM DTT fresh.
6. Microfuge.
7. Eppendorf clear.
8. SpeedVac™ concentrator.
9. 25 mM Ambic in Milli-Qwater.
10. 660 mM iodoacetic acid (IAA) prepared in 25 mM Ambic.
11. Citrate/citric acid buffer, pH 5.8 (100 mM citrate, 100 mM
citric acid).
12. 100 mM Tris–HCl, pH 5.5.
13. Thermostatic bath.
Fe Nanoparticles
1. Fe nanoparticles.
2. Miller-GP
® filter unit with a size of 0.22 μm.
3. 500 mM DTT fresh.
4. Microfuge.
5. Eppendorf clear.
6. SpeedVac™ concentrator.
7. 25 mM Ambic in Milli-Q water.
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