Fig. 2 Differences between total protein identification techniques such as shotgun, peptide selected searching
techniques such as SRM/MRM, or a massive and quantitative SRM search such as SWATH-MS
Fig. 3 Schematic representation of the strategies to build the spectral library in SWATH-MS analysis. (A) The
spectral library is obtained from a simple DDA experiment. It contains all the information regarding a given
identified peptide (such as tR, precursor m/z, and MS/MS spectra). (B) Once the library is performed, the
samples run in SWATH mode
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techniques such as SRM/MRM, or a massive and quantitative SRM search such as SWATH-MS
Fig. 3 Schematic representation of the strategies to build the spectral library in SWATH-MS analysis. (A) The
spectral library is obtained from a simple DDA experiment. It contains all the information regarding a given
identified peptide (such as tR, precursor m/z, and MS/MS spectra). (B) Once the library is performed, the
samples run in SWATH mode
108
Maria del Pilar Chantada-Va ´ zquez et al.