2.2 Dissolved
Organic Carbon
1. 24 ml glass autosampler vials with screw caps.
2. Oven (450
C).
3. Concentrated hydrochloric acid (37% HCl).
4. Ultrapure water (e.g., Milli-Q).
5. 0.7 μm glass fiber filters (25 mm Ø) and Swinnex filter holders.
6. Disposable 20 ml sterile syringes.
7. Potassium hydrogen phthalate (C 8 H 5 KO 4 ).
8. Spatulas.
9. Analytical balance.
10. 100–1000 ml volumetric flasks.
11. Adjustable pipettes and pipette tips.
12. Ultrasonicator bath.
13. Plastic film (e.g., Parafilm).
14. Refrigerator and freezer.
15. Carbon analyzer with autosampler and instrument-specific carrier gas and oxidizing agents.
2.3 Particulate
Organic Carbon
1. 0.7 μm glass fiber filters (e.g., 25 mm Ø).
2. Concentrated hydrochloric acid (37% HCl).
3. Forceps.
4. Ultrapure water (e.g., Milli-Q).
5. Oven (450
C).
6. Vacuum filtration device with a vacuum pump.
7. Small (30 mm Ø) disposable petri dishes.
8. Aluminum foil circles (30 mm).
9. Handheld plier suitable for making small balls out of aluminum.
10. 96-well plates.
11. Bunsen burner.
12. Ethanol (96%).
13. Glycine (C 2 H 5 NO 2 ).
14. Spatulas.
15. Analytical balance.
16. 100 ml volumetric flask.
17. Adjustable pipettes and pipette tips.
18. Refrigerator.
19. CN elemental analyzer with autosampler and instrument-specific
carrier gas and oxidizing agents.
Determining Carbon Concentration
65
Organic Carbon
1. 24 ml glass autosampler vials with screw caps.
2. Oven (450
C).
3. Concentrated hydrochloric acid (37% HCl).
4. Ultrapure water (e.g., Milli-Q).
5. 0.7 μm glass fiber filters (25 mm Ø) and Swinnex filter holders.
6. Disposable 20 ml sterile syringes.
7. Potassium hydrogen phthalate (C 8 H 5 KO 4 ).
8. Spatulas.
9. Analytical balance.
10. 100–1000 ml volumetric flasks.
11. Adjustable pipettes and pipette tips.
12. Ultrasonicator bath.
13. Plastic film (e.g., Parafilm).
14. Refrigerator and freezer.
15. Carbon analyzer with autosampler and instrument-specific carrier gas and oxidizing agents.
2.3 Particulate
Organic Carbon
1. 0.7 μm glass fiber filters (e.g., 25 mm Ø).
2. Concentrated hydrochloric acid (37% HCl).
3. Forceps.
4. Ultrapure water (e.g., Milli-Q).
5. Oven (450
C).
6. Vacuum filtration device with a vacuum pump.
7. Small (30 mm Ø) disposable petri dishes.
8. Aluminum foil circles (30 mm).
9. Handheld plier suitable for making small balls out of aluminum.
10. 96-well plates.
11. Bunsen burner.
12. Ethanol (96%).
13. Glycine (C 2 H 5 NO 2 ).
14. Spatulas.
15. Analytical balance.
16. 100 ml volumetric flask.
17. Adjustable pipettes and pipette tips.
18. Refrigerator.
19. CN elemental analyzer with autosampler and instrument-specific
carrier gas and oxidizing agents.
Determining Carbon Concentration
65