Total FAME C13 normalized ¼
X
C4‐C24
Amount Measured FAME C i
Amount Measured FAME C13
 Amount Added FAME C13
2. Calculate the total FAME as a percent of the dry weight of the
sample. For the FAME analysis, the dry weight refers to the
weight after drying the sample overnight at 40
C in a vacuum
oven:
%TotalFAME ¼
Total FAME C13normalized
ODW sample
 100
3. To report or calculate the root-mean-square deviation
(RMSD) or the standard deviation (STDEV) of the samples,
use the following calculation:
RMS ¼ x m ¼ mean ¼
ffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffi
P n
1
x
n
0
B
B
@
1
C
C
A
2
v
u
u
u
u
u
t
RMS deviation ¼ σ ¼ stdev ¼
ffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffi ffi
P n
1
x i À x m
ð
Þ
2
n
v
u
u
u
t
where x m ¼ the root mean square of all  values in the set,
n ¼ number of samples in set, x i ¼ measured value from the set
4. To report or calculate the quality control metrics, use the
relative standard deviation (RSD) of the triplicates, and use
the following calculation (see Note 17):
RSD ¼
stdev
X mean
 100
where stdev ¼ RMS deviation, X mean ¼ the mean % total FAME
of the triplicates.
4 Notes
1. Each sample should be analyzed in triplicate at a minimum,
unless the amount of sample prohibits this. In addition, a quality
control (QC) biomass or oil standard should be included in
Total Fatty Acid Content Determination of Whole Microalgal Biomass Using In. . .
209