to volume with HPLC grade hexane, and mix well. Transfer the
C13:0ME-hexane solution into 1.5 mL GC vials, and seal immediately with PTFE/silicone/PTFE crimp caps. Take extra care
while transferring to limit evaporation, as it is very important to
maintain the recorded concentration. Record the date of preparation, concentration, and any other pertinent information on the
vials, and place upright in a laboratory freezer (À20
C) for
storage. Store sealed for up to 6 months.
2. The GLC 461C FAME standard from NuChek was prepared
specifically for use with microalgal FAME analysis and contains
a typical mix of microalgal FAMEs. If using another mix, use an
analytically certified standard for quantitation. Do not use
qualitative mixes or uncertified mixes.
3. To prepare the standards and create a stock of prediluted calibration mixes, tare a clean, 10 mL, class A, volumetric flask.
Score the neck of the 461C ampule using a GC column cutter
or glass scorer. Do not snap the top off yet. Warm the 461C neat
mix ampule with your hands to be sure that all the mix is in the
bottom of the vial and no compounds are crystallized in the top
of the vial before breaking off the top. Using a glass Pasteur
pipet, transfer the neat mix as quantitatively as possible to the
tared 10 mL, class A, volumetric flask. Keep the Pasteur pipet
and neat mix warm with your fingers to prevent the crystallization of saturated longer-chain fatty acids while transferring.
Record the weight to the nearest 0.1 mg. Bring to volume
with HPLC grade hexane, and mix well. Transfer approximately
1 mL of the mixture to the labeled 1.5 mL GC vials using a glass
Pasteur pipet, working quickly and carefully to avoid evaporation. Seal vials immediately with PTFE/silicone/PTFE crimp
caps. Record the date of preparation, concentration, and any
other pertinent information on the vials, and place upright in a
laboratory freezer (À20
C) for storage. Store sealed for up to
6 months.
3 Methods
Perform all work in a fume hood. Use solvent-compatible analytical
ware when completing all steps. Carefully follow all relevant chemical handling procedures.
3.1 Sample
Preparation
1. Label 1.5 mL GC vials using a permanent marker for each of
the samples and quality control samples to be analyzed
(see Note 1).
2. Record the weights of all labeled vials to the nearest 0.1 mg.
Written labels should be dry before the weight is recorded.
206
S. Van Wychen and L.M.L Laurens
C13:0ME-hexane solution into 1.5 mL GC vials, and seal immediately with PTFE/silicone/PTFE crimp caps. Take extra care
while transferring to limit evaporation, as it is very important to
maintain the recorded concentration. Record the date of preparation, concentration, and any other pertinent information on the
vials, and place upright in a laboratory freezer (À20
C) for
storage. Store sealed for up to 6 months.
2. The GLC 461C FAME standard from NuChek was prepared
specifically for use with microalgal FAME analysis and contains
a typical mix of microalgal FAMEs. If using another mix, use an
analytically certified standard for quantitation. Do not use
qualitative mixes or uncertified mixes.
3. To prepare the standards and create a stock of prediluted calibration mixes, tare a clean, 10 mL, class A, volumetric flask.
Score the neck of the 461C ampule using a GC column cutter
or glass scorer. Do not snap the top off yet. Warm the 461C neat
mix ampule with your hands to be sure that all the mix is in the
bottom of the vial and no compounds are crystallized in the top
of the vial before breaking off the top. Using a glass Pasteur
pipet, transfer the neat mix as quantitatively as possible to the
tared 10 mL, class A, volumetric flask. Keep the Pasteur pipet
and neat mix warm with your fingers to prevent the crystallization of saturated longer-chain fatty acids while transferring.
Record the weight to the nearest 0.1 mg. Bring to volume
with HPLC grade hexane, and mix well. Transfer approximately
1 mL of the mixture to the labeled 1.5 mL GC vials using a glass
Pasteur pipet, working quickly and carefully to avoid evaporation. Seal vials immediately with PTFE/silicone/PTFE crimp
caps. Record the date of preparation, concentration, and any
other pertinent information on the vials, and place upright in a
laboratory freezer (À20
C) for storage. Store sealed for up to
6 months.
3 Methods
Perform all work in a fume hood. Use solvent-compatible analytical
ware when completing all steps. Carefully follow all relevant chemical handling procedures.
3.1 Sample
Preparation
1. Label 1.5 mL GC vials using a permanent marker for each of
the samples and quality control samples to be analyzed
(see Note 1).
2. Record the weights of all labeled vials to the nearest 0.1 mg.
Written labels should be dry before the weight is recorded.
206
S. Van Wychen and L.M.L Laurens