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10.1038/nature04303
86. Ebert MS, Sharp PA (2010) MicroRNA sponges: progress and possibilities. RNA 16
(11):2043–2050. https://doi.org/10.1261/rna.2414110
87. Kuhn DE, Martin MM, Feldman DS, Terry AV Jr, Nuovo GJ, Elton TS (2008)
Experimental validation of miRNA targets. Methods 44(1):47–54. https://doi.org/10.1016/j.
ymeth.2007.09.005
88. Beitzinger M, Peters L, Zhu JY, Kremmer E, Meister G (2007) Identification of human
microRNA targets from isolated argonaute protein complexes. RNA Biol 4(2):76–84
89. Licatalosi DD, Mele A, Fak JJ, Ule J, Kayikci M, Chi SW, Clark TA, Schweitzer AC, Blume
JE, Wang X, Darnell JC, Darnell RB (2008) HITS-CLIP yields genome-wide insights into brain
alternative RNA processing. Nature 456(7221):464–469. https://doi.org/10.1038/nature07488
90. Hafner M, Landthaler M, Burger L, Khorshid M, Hausser J, Berninger P, Rothballer A, Ascano
M Jr, Jungkamp AC, Munschauer M, Ulrich A, Wardle GS, Dewell S, Zavolan M, Tuschl T
(2010) Transcriptome-wide identification of RNA-binding protein and microRNA target sites
by PAR-CLIP. Cell 141(1):129–141. https://doi.org/10.1016/j.cell.2010.03.009
91. Danan C, Manickavel S, Hafner M (2016) PAR-CLIP: a method for transcriptome-wide
identification of RNA binding protein interaction sites. Methods Mol Biol 1358:153–173.
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science.1090095
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photosensitized RNA-protein crosslinking in mammalian cells. Biochem Biophys Res
Commun 141(2):847–854
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Escherichia coli RNAs. Biochem Biophys Res Commun 166(1):29–37
95. Kishore S, Jaskiewicz L, Burger L, Hausser J, Khorshid M, Zavolan M (2011) A quantitative
analysis of CLIP methods for identifying binding sites of RNA-binding proteins. Nat Methods 8
(7):559–564. https://doi.org/10.1038/nmeth.1608
96. Baigude H, Ahsanullah LZ, Zhou Y, Rana TM (2012) miR-TRAP: a benchtop chemical
biology strategy to identify microRNA targets. Angew Chem Int Ed Engl 51(24):5880–5883.
https://doi.org/10.1002/anie.201201512
97. Cambronne XA, Shen R, Auer PL, Goodman RH (2012) Capturing microRNA targets using an
RNA-induced silencing complex (RISC)-trap approach. Proc Natl Acad Sci U S A 109
(50):20473–20478. https://doi.org/10.1073/pnas.1218887109
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C. Dell’Aversana et al.