4. Ho SM, Johnson A, Tarapore P, Janakiram V, Zhang X, Leung YK (2012) Environmental
epigenetics and its implication on disease risk and health outcomes. ILAR J 53(3–4):289–305.
https://doi.org/10.1093/ilar.53.3-4.289
5. Thomas ML, Marcato P (2018) Epigenetic modifications as biomarkers of tumor development,
therapy response, and recurrence across the cancer care continuum. Cancers (Basel) 10(4).
https://doi.org/10.3390/cancers10040101
6. Vardabasso C, Gaspar-Maia A, Hasson D, Punzeler S, Valle-Garcia D, Straub T, Keilhauer EC,
Strub T, Dong J, Panda T, Chung CY, Yao JL, Singh R, Segura MF, Fontanals-Cirera B,
Verma A, Mann M, Hernando E, Hake SB, Bernstein E (2015) Histone variant H2A.Z.2
mediates proliferation and drug sensitivity of malignant melanoma. Mol Cell 59(1):75–88.
https://doi.org/10.1016/j.molcel.2015.05.009
7. Jia M, Jansen L, Walter V, Tagscherer K, Roth W, Herpel E, Kloor M, Blaker H, ChangClaude J, Brenner H, Hoffmeister M (2016) No association of CpG island methylator phenotype
and colorectal cancer survival: population-based study. Br J Cancer 115(11):1359–1366.
https://doi.org/10.1038/bjc.2016.361
8. Ullman EF, Kirakossian H, Singh S, Wu ZP, Irvin BR, Pease JS, Switchenko AC, Irvine JD,
Dafforn A, Skold CN et al (1994) Luminescent oxygen channeling immunoassay: measurement
of particle binding kinetics by chemiluminescence. Proc Natl Acad Sci U S A 91
(12):5426–5430
9. Yasgar A, Jadhav A, Simeonov A, Coussens NP (2016) AlphaScreen-based assays: ultra-highthroughput screening for small-molecule inhibitors of challenging enzymes and protein-protein
interactions. Methods Mol Biol 1439:77–98. https://doi.org/10.1007/978-1-4939-3673-1_5
10. Wigle TJ, Herold JM, Senisterra GA, Vedadi M, Kireev DB, Arrowsmith CH, Frye SV, Janzen
WP (2010) Screening for inhibitors of low-affinity epigenetic peptide-protein interactions: an
AlphaScreen-based assay for antagonists of methyl-lysine binding proteins. J Biomol Screen 15
(1):62–71. https://doi.org/10.1177/1087057109352902
11. Prabhu L, Chen L, Wei H, Demir O, Safa A, Zeng L, Amaro RE, O’Neil BH, Zhang ZY, Lu T
(2017) Development of an AlphaLISA high throughput technique to screen for small molecule
inhibitors targeting protein arginine methyltransferases. Mol Biosyst 13(12):2509–2520. https://
doi.org/10.1039/c7mb00391a
12. Scarano S, Scuffi C, Mascini M, Minunni M (2011) Surface plasmon resonance imaging-based
sensing for anti-bovine immunoglobulins detection in human milk and serum. Anal Chim Acta
707(1–2):178–183. https://doi.org/10.1016/j.aca.2011.09.012
13. Kim D, Lee IS, Jung JH, Yang SI (1999) Psammaplin A, a natural bromotyrosine derivative
from a sponge, possesses the antibacterial activity against methicillin-resistant Staphylococcus
aureus and the DNA gyrase-inhibitory activity. Arch Pharm Res 22(1):25–29
14. Duff MR Jr, Grubbs J, Howell EE (2011) Isothermal titration calorimetry for measuring
macromolecule-ligand affinity. J Vis Exp (55). https://doi.org/10.3791/2796
15. Holdgate G (2009) Isothermal titration calorimetry and differential scanning calorimetry.
Methods Mol Biol 572:101–133. https://doi.org/10.1007/978-1-60761-244-5_7
16. Jerabek-Willemsen M, Wienken CJ, Braun D, Baaske P, Duhr S (2011) Molecular interaction
studies using microscale thermophoresis. Assay Drug Dev Technol 9(4):342–353. https://doi.
org/10.1089/adt.2011.0380
17. Zillner K, Jerabek-Willemsen M, Duhr S, Braun D, Langst G, Baaske P (2012) Microscale
thermophoresis as a sensitive method to quantify protein: nucleic acid interactions in solution.
Methods Mol Biol 815:241–252. https://doi.org/10.1007/978-1-61779-424-7_18
18. Alpatov R, Lesch BJ, Nakamoto-Kinoshita M, Blanco A, Chen S, Stutzer A, Armache KJ,
Simon MD, Xu C, Ali M, Murn J, Prisic S, Kutateladze TG, Vakoc CR, Min J, Kingston RE,
Fischle W, Warren ST, Page DC, Shi Y (2014) A chromatin-dependent role of the fragile X
mental retardation protein FMRP in the DNA damage response. Cell 157(4):869–881. https://
doi.org/10.1016/j.cell.2014.03.040
19. Josling GA, Petter M, Oehring SC, Gupta AP, Dietz O, Wilson DW, Schubert T, Langst G,
Gilson PR, Crabb BS, Moes S, Jenoe P, Lim SW, Brown GV, Bozdech Z, Voss TS, Duffy MF
Advanced Assays in Epigenetics
555
epigenetics and its implication on disease risk and health outcomes. ILAR J 53(3–4):289–305.
https://doi.org/10.1093/ilar.53.3-4.289
5. Thomas ML, Marcato P (2018) Epigenetic modifications as biomarkers of tumor development,
therapy response, and recurrence across the cancer care continuum. Cancers (Basel) 10(4).
https://doi.org/10.3390/cancers10040101
6. Vardabasso C, Gaspar-Maia A, Hasson D, Punzeler S, Valle-Garcia D, Straub T, Keilhauer EC,
Strub T, Dong J, Panda T, Chung CY, Yao JL, Singh R, Segura MF, Fontanals-Cirera B,
Verma A, Mann M, Hernando E, Hake SB, Bernstein E (2015) Histone variant H2A.Z.2
mediates proliferation and drug sensitivity of malignant melanoma. Mol Cell 59(1):75–88.
https://doi.org/10.1016/j.molcel.2015.05.009
7. Jia M, Jansen L, Walter V, Tagscherer K, Roth W, Herpel E, Kloor M, Blaker H, ChangClaude J, Brenner H, Hoffmeister M (2016) No association of CpG island methylator phenotype
and colorectal cancer survival: population-based study. Br J Cancer 115(11):1359–1366.
https://doi.org/10.1038/bjc.2016.361
8. Ullman EF, Kirakossian H, Singh S, Wu ZP, Irvin BR, Pease JS, Switchenko AC, Irvine JD,
Dafforn A, Skold CN et al (1994) Luminescent oxygen channeling immunoassay: measurement
of particle binding kinetics by chemiluminescence. Proc Natl Acad Sci U S A 91
(12):5426–5430
9. Yasgar A, Jadhav A, Simeonov A, Coussens NP (2016) AlphaScreen-based assays: ultra-highthroughput screening for small-molecule inhibitors of challenging enzymes and protein-protein
interactions. Methods Mol Biol 1439:77–98. https://doi.org/10.1007/978-1-4939-3673-1_5
10. Wigle TJ, Herold JM, Senisterra GA, Vedadi M, Kireev DB, Arrowsmith CH, Frye SV, Janzen
WP (2010) Screening for inhibitors of low-affinity epigenetic peptide-protein interactions: an
AlphaScreen-based assay for antagonists of methyl-lysine binding proteins. J Biomol Screen 15
(1):62–71. https://doi.org/10.1177/1087057109352902
11. Prabhu L, Chen L, Wei H, Demir O, Safa A, Zeng L, Amaro RE, O’Neil BH, Zhang ZY, Lu T
(2017) Development of an AlphaLISA high throughput technique to screen for small molecule
inhibitors targeting protein arginine methyltransferases. Mol Biosyst 13(12):2509–2520. https://
doi.org/10.1039/c7mb00391a
12. Scarano S, Scuffi C, Mascini M, Minunni M (2011) Surface plasmon resonance imaging-based
sensing for anti-bovine immunoglobulins detection in human milk and serum. Anal Chim Acta
707(1–2):178–183. https://doi.org/10.1016/j.aca.2011.09.012
13. Kim D, Lee IS, Jung JH, Yang SI (1999) Psammaplin A, a natural bromotyrosine derivative
from a sponge, possesses the antibacterial activity against methicillin-resistant Staphylococcus
aureus and the DNA gyrase-inhibitory activity. Arch Pharm Res 22(1):25–29
14. Duff MR Jr, Grubbs J, Howell EE (2011) Isothermal titration calorimetry for measuring
macromolecule-ligand affinity. J Vis Exp (55). https://doi.org/10.3791/2796
15. Holdgate G (2009) Isothermal titration calorimetry and differential scanning calorimetry.
Methods Mol Biol 572:101–133. https://doi.org/10.1007/978-1-60761-244-5_7
16. Jerabek-Willemsen M, Wienken CJ, Braun D, Baaske P, Duhr S (2011) Molecular interaction
studies using microscale thermophoresis. Assay Drug Dev Technol 9(4):342–353. https://doi.
org/10.1089/adt.2011.0380
17. Zillner K, Jerabek-Willemsen M, Duhr S, Braun D, Langst G, Baaske P (2012) Microscale
thermophoresis as a sensitive method to quantify protein: nucleic acid interactions in solution.
Methods Mol Biol 815:241–252. https://doi.org/10.1007/978-1-61779-424-7_18
18. Alpatov R, Lesch BJ, Nakamoto-Kinoshita M, Blanco A, Chen S, Stutzer A, Armache KJ,
Simon MD, Xu C, Ali M, Murn J, Prisic S, Kutateladze TG, Vakoc CR, Min J, Kingston RE,
Fischle W, Warren ST, Page DC, Shi Y (2014) A chromatin-dependent role of the fragile X
mental retardation protein FMRP in the DNA damage response. Cell 157(4):869–881. https://
doi.org/10.1016/j.cell.2014.03.040
19. Josling GA, Petter M, Oehring SC, Gupta AP, Dietz O, Wilson DW, Schubert T, Langst G,
Gilson PR, Crabb BS, Moes S, Jenoe P, Lim SW, Brown GV, Bozdech Z, Voss TS, Duffy MF
Advanced Assays in Epigenetics
555