minimizing the recovery of non-specific targets [96]. The pull-down method significantly enhances the enrichment of a specific target sequence for analysis via qRT-PCR.
Most notably, miR-TRAP could be combined with HTS, providing the rapid identification of canonical but more importantly also of non-canonical and thus unpredictable
regulatory miRNAs:mRNA networks in a cellular context [97]. This enhanced capacity
has exceptional potential to discover novel mRNA targets for a miRNA of interest,
including those that are transient or in low abundance. Accordingly, miR-TRAP is
advantageous for the rapid identification of miRNAs at low false-positive and presumably low false-negative rates. Moreover, this approach is feasible and independent of
genetic manipulations, allowing the analysis of several primary samples. Finally,
miR-TRAP is a powerful, rapid, cost-effective and easy-to-handle tool that promotes
the study of miRNA-dependent regulation in different diseases.
Fig. 9 Schematic representation of CLIP and PAR-CLIP procedures
Advanced Assays in Epigenetics
553
Most notably, miR-TRAP could be combined with HTS, providing the rapid identification of canonical but more importantly also of non-canonical and thus unpredictable
regulatory miRNAs:mRNA networks in a cellular context [97]. This enhanced capacity
has exceptional potential to discover novel mRNA targets for a miRNA of interest,
including those that are transient or in low abundance. Accordingly, miR-TRAP is
advantageous for the rapid identification of miRNAs at low false-positive and presumably low false-negative rates. Moreover, this approach is feasible and independent of
genetic manipulations, allowing the analysis of several primary samples. Finally,
miR-TRAP is a powerful, rapid, cost-effective and easy-to-handle tool that promotes
the study of miRNA-dependent regulation in different diseases.
Fig. 9 Schematic representation of CLIP and PAR-CLIP procedures
Advanced Assays in Epigenetics
553