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structure of TET2-DNA complex: insight into TET-mediated 5mC oxidation. Cell 155
(7):1545–1555
122. Fu Y, Luo GZ, Chen K, Deng X, Yu M, Han D, Hao Z, Liu J, Lu X, Dore LC, Weng X, Ji Q,
Mets L, He C (2015) N6-methyldeoxyadenosine marks active transcription start sites in
Chlamydomonas. Cell 161(4):879–892. https://doi.org/10.1016/j.cell.2015.04.010
123. Nakano K, Shiroma A, Shimoji M, Tamotsu H, Ashimine N, Ohki S, Shinzato M, Minami M,
Nakanishi T, Teruya K, Satou K, Hirano T (2017) Advantages of genome sequencing by longread sequencer using SMRT technology in medical area. Hum Cell 30(3):149–161. https://doi.
org/10.1007/s13577-017-0168-8
124. Rhoads A, Au KF (2015) PacBio sequencing and its applications. Genomics Proteomics
Bioinformatics 13(5):278–289. https://doi.org/10.1016/j.gpb.2015.08.002
125. Clark TA, Lu X, Luong K, Dai Q, Boitano M, Turner SW, He C, Korlach J (2013) Enhanced
5-methylcytosine detection in single-molecule, real-time sequencing via Tet1 oxidation. BMC
Biol 11:4. https://doi.org/10.1186/1741-7007-11-4
126. Chavez L, Huang Y, Luong K, Agarwal S, Iyer LM, Pastor WA, Hench VK, Frazier-Bowers
SA, Korol E, Liu S, Tahiliani M, Wang Y, Clark TA, Korlach J, Pukkila PJ, Aravind L, Rao A
(2014) Simultaneous sequencing of oxidized methylcytosines produced by TET/JBP
dioxygenases in Coprinopsis cinerea. Proc Natl Acad Sci U S A 111(48):E5149–E5158.
https://doi.org/10.1073/pnas.1419513111
127. Niedringhaus TP, Milanova D, Kerby MB, Snyder MP, Barron AE (2011) Landscape of nextgeneration sequencing technologies. Anal Chem 83(12):4327–4341. https://doi.org/10.1021/
ac2010857
128. Wescoe ZL, Schreiber J, Akeson M (2014) Nanopores discriminate among five C5-cytosine
variants in DNA. J Am Chem Soc 136(47):16582–16587. https://doi.org/10.1021/ja508527b
129. Rand AC, Jain M, Eizenga JM, Musselman-Brown A, Olsen HE, Akeson M, Paten B (2017)
Mapping DNA methylation with high-throughput nanopore sequencing. Nat Methods 14
(4):411–413. https://doi.org/10.1038/nmeth.4189
130. Weirather JL, de Cesare M, Wang Y, Piazza P, Sebastiano V, Wang XJ, Buck D, Au KF
(2017) Comprehensive comparison of Pacific Biosciences and Oxford Nanopore Technologies
and their applications to transcriptome analysis. F1000Res 6:100. https://doi.org/10.12688/
f1000research.10571.2
Experimental Methodologies for Detection and Mapping of Epigenetic DNA Marks
521