(via ANOVA), there was an observed difference in the amounts of respiration and
production between the control system and the solvent addition system on each
measurement day. In the case of acetone addition, significantly higher values relative
to the control system were shown for the respiration amount on the 9th day after
addition and the production amount on the 7–9th days after addition. In this way,
because the pesticide had very low solubility in water, it was necessary to dissolve it
in organic solvents, such as acetone, but it became clear that the organic solvent itself
caused an increase in the amounts of production and respiration.
7.8.4 Methanol
When methanol is used as a solvent for a pesticide that is added to the microcosm,
more than three times the respiration and production amounts are measured in the
acetone addition system than are measured in the control system. Treated statistically
(via ANOVA), there was an observed difference in the amounts of respiration and
production between the control system and the solvent addition system on each
measurement day. In the case of acetone addition, significantly higher values relative
to the control system were shown for the respiration amount on the 9th day after
addition and the production amount on the 7–9th days after addition. In this way,
because the pesticide had very low solubility in water, it was necessary to dissolve it
in organic solvents, such as acetone, but it became clear that the organic solvent itself
caused an increase in the amounts of production and respiration.
7.8.5 Phenol
The effect of phenol was investigated and reported by Dr. Sugiura. Phenol was
added at the time microcosm cultivation began (day 0), and the microorganism
population and amounts of respiration and production were tracked over time. The
amount of production became larger than that of the control system, but, with the
addition of phenol, the amount of respiration was not observed to change with the
addition of phenol when compared with the control system. The m-NOEC of phenol
was estimated to be 5 mg/L (Sugiura 1992).
7.8.6 2.4-Dichlorophenol
2,4-Dichlorophenol is used as an organic, phosphorus-based insecticide and sterilizer, a phenoxy-based weed killer, and for herbicidal raw materials, and it may be
generated by the resolution of a pesticide. The effect of 2,4-dichlorophenol was
investigated and reported by Dr. Sugiura, and it was added to the microcosm when
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K. Murakami et al.
production between the control system and the solvent addition system on each
measurement day. In the case of acetone addition, significantly higher values relative
to the control system were shown for the respiration amount on the 9th day after
addition and the production amount on the 7–9th days after addition. In this way,
because the pesticide had very low solubility in water, it was necessary to dissolve it
in organic solvents, such as acetone, but it became clear that the organic solvent itself
caused an increase in the amounts of production and respiration.
7.8.4 Methanol
When methanol is used as a solvent for a pesticide that is added to the microcosm,
more than three times the respiration and production amounts are measured in the
acetone addition system than are measured in the control system. Treated statistically
(via ANOVA), there was an observed difference in the amounts of respiration and
production between the control system and the solvent addition system on each
measurement day. In the case of acetone addition, significantly higher values relative
to the control system were shown for the respiration amount on the 9th day after
addition and the production amount on the 7–9th days after addition. In this way,
because the pesticide had very low solubility in water, it was necessary to dissolve it
in organic solvents, such as acetone, but it became clear that the organic solvent itself
caused an increase in the amounts of production and respiration.
7.8.5 Phenol
The effect of phenol was investigated and reported by Dr. Sugiura. Phenol was
added at the time microcosm cultivation began (day 0), and the microorganism
population and amounts of respiration and production were tracked over time. The
amount of production became larger than that of the control system, but, with the
addition of phenol, the amount of respiration was not observed to change with the
addition of phenol when compared with the control system. The m-NOEC of phenol
was estimated to be 5 mg/L (Sugiura 1992).
7.8.6 2.4-Dichlorophenol
2,4-Dichlorophenol is used as an organic, phosphorus-based insecticide and sterilizer, a phenoxy-based weed killer, and for herbicidal raw materials, and it may be
generated by the resolution of a pesticide. The effect of 2,4-dichlorophenol was
investigated and reported by Dr. Sugiura, and it was added to the microcosm when
112
K. Murakami et al.