3 Isolation and Storage of Soil Nematodes
3.1 Isolation
Techniques of Soil
Nematodes Depend On
l
Nematode type: ectoparasite, endoparasite, soil nematode.
l
Nematode size: small, large.
l
Nematode movement: slow, rapid.
l
Nematode state: dead, alive.
Each technique has its advantages and limitations.
3.2 Direct Method
3.2.1 Baermann Funnel
Technique [158] (Fig. 7)
Materials Required
l
Glass funnel.
l
Hose pipe/rubber tube.
l
Ring stand.
l
Wire screen/nylon mesh screen.
l
Stem with a squeezer clip/clamp.
l
Tissue paper.
l
Soil sample.
l
Tap water.
l
Petri plate/watch glass.
Fig. 7 Baermann funnel technique
Isolation and Storage of Soil Nematodes
59