l
Gas chromatography (GC).
l
HP 6890 GC-5973 MS system.
l
Eclipse 4660 purge.
l
Trap sample concentrator.
l
HP-624 capillary column.
l
Quadrupole mass spectrometer.
3.1.2 Procedure
1. Take 125-ml flask.
2. Place last-instar larvae (Galleria mellonella and Tenebrio molitor) within the flask.
3. Flow into the flask a current of air (10% oxygen, 90% nitrogen)
and out through a thermal desorption tube at nearly 104 ml/
min flow rate.
Note: Experiments are carried out in sets, with a vacant
control flask being run every time.
4. Transfer the substances from the thermal desorption tube to
the HP 6890 GC-5973 MS system with an Eclipse 4660 purge
and trap sample fitted with an air tube desorber accessory.
5. Desorb the tubes at 200
C for 15 min and transfer through a
helium flow to an inner trap detained at room temperature.
6. Heat the inner trap to 200
C subsequent to desorption.
7. Bring this trap in line through the GC carrier gas flow as the
trap reaches 180
C.
8. Take the trap offline and run it through a bake out process.
9. Flow the sample to a GC through a transfer line maintained at
120
C, and it moves in a split-splitless injector apprehended at
200
C.
10. Operate the injector in split mode of split ratio at 30:1, later
install a 1 mm liner to augment chromatographic resolution.
11. Attain separation with HP-624 capillary column
(30 m  0.320 mm) in which a volumetric flow of 1 ml/min
is upheld by electronic pressure control.
12. Clutch the transfer line to the mass spectrometer at 200
C, the
ion source at 250
C, and the quadrupole at 100
C. The mass
spectrometer is fitted by an electron impact source.
13. Establish electron energy to 70 eV in order to acquire the best
conceivable library spectrum matches.
14. Operate the quadrupole mass spectrometer with full width at
0.65 m/z half maximum.
15. Authenticate mass calibration each week.
16. Ramp the GC oven from 30 to 260
C and run for 42 min.
Volatile Cues
37
Gas chromatography (GC).
l
HP 6890 GC-5973 MS system.
l
Eclipse 4660 purge.
l
Trap sample concentrator.
l
HP-624 capillary column.
l
Quadrupole mass spectrometer.
3.1.2 Procedure
1. Take 125-ml flask.
2. Place last-instar larvae (Galleria mellonella and Tenebrio molitor) within the flask.
3. Flow into the flask a current of air (10% oxygen, 90% nitrogen)
and out through a thermal desorption tube at nearly 104 ml/
min flow rate.
Note: Experiments are carried out in sets, with a vacant
control flask being run every time.
4. Transfer the substances from the thermal desorption tube to
the HP 6890 GC-5973 MS system with an Eclipse 4660 purge
and trap sample fitted with an air tube desorber accessory.
5. Desorb the tubes at 200
C for 15 min and transfer through a
helium flow to an inner trap detained at room temperature.
6. Heat the inner trap to 200
C subsequent to desorption.
7. Bring this trap in line through the GC carrier gas flow as the
trap reaches 180
C.
8. Take the trap offline and run it through a bake out process.
9. Flow the sample to a GC through a transfer line maintained at
120
C, and it moves in a split-splitless injector apprehended at
200
C.
10. Operate the injector in split mode of split ratio at 30:1, later
install a 1 mm liner to augment chromatographic resolution.
11. Attain separation with HP-624 capillary column
(30 m  0.320 mm) in which a volumetric flow of 1 ml/min
is upheld by electronic pressure control.
12. Clutch the transfer line to the mass spectrometer at 200
C, the
ion source at 250
C, and the quadrupole at 100
C. The mass
spectrometer is fitted by an electron impact source.
13. Establish electron energy to 70 eV in order to acquire the best
conceivable library spectrum matches.
14. Operate the quadrupole mass spectrometer with full width at
0.65 m/z half maximum.
15. Authenticate mass calibration each week.
16. Ramp the GC oven from 30 to 260
C and run for 42 min.
Volatile Cues
37