6. Add to the EPN exposure, treatment chambers that contain
autoclaved dampened sand, the EPN‐infected cadavers. Interconnect these chambers with plant chambers using a sand-filled
glass arm.
7. Expose plants in the control to headspace chemical compounds
either from G. mellonella in sand that is thawed and freeze‐
killed (control carcass), or only sand.
8. Separate roots of plants in every treated chambers from the
exposure‐source chambers using 400 mesh film having a distance of 28 cm.
9. Expose the plants to the several treatments for 48 h earlier to
obtain any measurements and uninterruptedly throughout the
period of the sampling process.
2.3 Collection and
Assaying of EPN
Volatiles
1. Collect volatile cues discharged by cadavers infected with EPN
by dynamic headspace sampling.
2. Analyze through gas chromatography coupled to mass spectrometry (GC/MS).
Table 1
(continued)
Nematode species
Chemical attractant
References
Nonanal
[143]
Octanal
Decanal
ß-Caryophyllene
α-Pinene
Bornyl acetate
Borneol
2,4-Di-tetra-butylphenol
2-Ethyl-hexanol
Terpinolene
H. megidis
(E)-β-Farnesene
[147]
(E)-Nerolidol
ß-Caryophyllene
[143]
H. indica
Geijerene
[145]
Pregeijerene
Tritrophic Interactions
35