3. Observe the behavior of the IJs under a dissecting microscope
with 45Â magnification.
3.2 Attachment Rate
[134]
Nematode foraging strategies are assessed by the use of a mobile
host (G. mellonella) compared to a sit-and-wait forager (ambusher)
and an active forager (cruisers).
1. Sprinkle 0.5 g of sand on a petri dish lined with moist filter
paper.
2. Add 1000 IJs/200 μl and allow for 15 min for the dispersal of
nematode and add one Galleria larva.
3. Using a probe, the larva should crawl continuously.
4. Remove the larva after 10 min and wash with distilled water.
5. Using a dissecting microscope, count the number of IJs.
3.3 Effect of
Substrate [134]
1. Fill 2 g of moist sand in a petri dish.
2. Add 50 IJs in 60 μl of water followed by a single G. mellonella
larva.
3. Dissect the dead larvae after 3 days and then digest in pepsin
solution.
4. Count the nematodes.
3.4 Host Finding
[134]
The potential of the IJs to detect and infect a sedentary host at
various soil depths is analyzed.
1. Fill a 15-cm plastic container with sandy loam soil up to 10 cm.
Fig. 1 Nictating infective juvenile of S. siamkayai
Foraging Assays
29
with 45Â magnification.
3.2 Attachment Rate
[134]
Nematode foraging strategies are assessed by the use of a mobile
host (G. mellonella) compared to a sit-and-wait forager (ambusher)
and an active forager (cruisers).
1. Sprinkle 0.5 g of sand on a petri dish lined with moist filter
paper.
2. Add 1000 IJs/200 μl and allow for 15 min for the dispersal of
nematode and add one Galleria larva.
3. Using a probe, the larva should crawl continuously.
4. Remove the larva after 10 min and wash with distilled water.
5. Using a dissecting microscope, count the number of IJs.
3.3 Effect of
Substrate [134]
1. Fill 2 g of moist sand in a petri dish.
2. Add 50 IJs in 60 μl of water followed by a single G. mellonella
larva.
3. Dissect the dead larvae after 3 days and then digest in pepsin
solution.
4. Count the nematodes.
3.4 Host Finding
[134]
The potential of the IJs to detect and infect a sedentary host at
various soil depths is analyzed.
1. Fill a 15-cm plastic container with sandy loam soil up to 10 cm.
Fig. 1 Nictating infective juvenile of S. siamkayai
Foraging Assays
29