2.3.1 Materials Required
l
Black vine weevil (BVW).
l
Composted green waste (CGW).
l
H. bacteriophora UWS 1.
l
Plastic pots (2 L).
l
Peat, bark, coir, and peat blends.
l
Tap water.
2.3.2 Procedure
1. Fill plastic pots (13 cm height, 16 cm diameter, 201 cm
2
surface area) with 10% peat, bark, coir, and peat blends.
2. Transplant 20% CGW.
3. Place eight third-instar BVW larvae all over the base of each
plant 4 h before the start of an experiment.
4. Substitute the larvae that had not entered into the substrates
within 2 h.
5. Smear H. bacteriophora as an inundate at three concentrations
(1) low (1.2 Â 10
9 IJs/ha), (2) medium (2.5 Â 10
9 IJs/ha),
and (3) high (5.0 Â 10
9 IJs/ha).
6. Apply the EPN suspensions in 50 ml of tap water per pot trailed
by another 50 ml of water to wash the EPN into the substrates.
7. Treat the control pots with 100 ml water only.
8. Arrange the pots in a random block design with 10 pots per
treatment.
9. Sample the pots destructively after 2 weeks.
10. Record the EPN application and the number of
surviving BVW.
11. During the experiment, maintain the air temperature in the
greenhouse 14.5–28.4 ºC (average 19.5 ºC).
12. Conduct the entire experiment twice.
158
Application Efficacy Against Insects in Other Than Soil Habitats
l
Black vine weevil (BVW).
l
Composted green waste (CGW).
l
H. bacteriophora UWS 1.
l
Plastic pots (2 L).
l
Peat, bark, coir, and peat blends.
l
Tap water.
2.3.2 Procedure
1. Fill plastic pots (13 cm height, 16 cm diameter, 201 cm
2
surface area) with 10% peat, bark, coir, and peat blends.
2. Transplant 20% CGW.
3. Place eight third-instar BVW larvae all over the base of each
plant 4 h before the start of an experiment.
4. Substitute the larvae that had not entered into the substrates
within 2 h.
5. Smear H. bacteriophora as an inundate at three concentrations
(1) low (1.2 Â 10
9 IJs/ha), (2) medium (2.5 Â 10
9 IJs/ha),
and (3) high (5.0 Â 10
9 IJs/ha).
6. Apply the EPN suspensions in 50 ml of tap water per pot trailed
by another 50 ml of water to wash the EPN into the substrates.
7. Treat the control pots with 100 ml water only.
8. Arrange the pots in a random block design with 10 pots per
treatment.
9. Sample the pots destructively after 2 weeks.
10. Record the EPN application and the number of
surviving BVW.
11. During the experiment, maintain the air temperature in the
greenhouse 14.5–28.4 ºC (average 19.5 ºC).
12. Conduct the entire experiment twice.
158
Application Efficacy Against Insects in Other Than Soil Habitats