Ansari SA, Husain Q (2012) Potential applications of enzymes immobilized on/in nano materials: a
review. Biotechnol Adv 30(3):512–523. https://doi.org/10.1016/j.biotechadv.2011.09.005
Arca-Ramos A, Ammann EM, Gasser CA, Nastold P, Eibes G, Feijoo G, Lema JM, Moreira MT,
Corvini PF-X (2016) Assessing the use of nanoimmobilized laccases to remove micropollutants
from wastewater. Environ Sci Pollut Res 23(4):3217–3228. https://doi.org/10.1007/s11356015-5564-6
Ardao I, Magnin D, Agathos SN (2015) Bioinspired production of magnetic laccase-biotitania
particles for the removal of endocrine disrupting chemicals. Biotechnol Bioeng 112
(10):1986–1996. https://doi.org/10.1002/bit.25612
Arora PK, Srivastava A, Singh VP (2010) Application of monooxygenases in dehalogenation,
desulphurization, denitrification and hydroxylation of aromatic compounds. J Bioremed
Biodegr 1(3):1000112. https://doi.org/10.4172/2155-6199.1000112
Ba S, Haroune L, Cruz-Morató C, Jacquet C, Touahar IE, Bellenger J-P, Legault CY, Jones JP,
Cabana H (2014) Synthesis and characterization of combined cross-linked laccase and tyrosinase aggregates transforming acetaminophen as a model phenolic compound in wastewaters.
Sci Total Environ 487(1):748–755. https://doi.org/10.1016/j.scitotenv.2013.10.004
Badgujar KC, Sasaki T, Bhanage BM (2015) Synthesis of lipase nano-bio-conjugates as an efficient
biocatalyst: characterization and activity–stability studies with potential biocatalytic applications. RSC Adv 5(68):55238–55251. https://doi.org/10.1039/C5RA10032A
Bansal N, Kanwar SS (2013) Peroxidase(s) in environment protection. Sci World J 2013:714639.
https://doi.org/10.1155/2013/714639
Bilal M, Iqbal HMN, Hu H, Wang W, Zhang X (2017) Development of horseradish peroxidasebased cross-linked enzyme aggregates and their environmental exploitation for bioremediation
purposes. J Environ Manag 188:137–143. https://doi.org/10.1016/j.jenvman.2016.12.015
Boujakhrout A, Jimenez-Falcao S, Martínez-Ruiz P, Sánchez A, Díez P, Pingarrón JM, Villalonga
R (2016) Novel reduced graphene oxide–glycol chitosan nanohybrid for the assembly of an
amperometric enzyme biosensor for phenols. Analyst 141(13):4162–4169. https://doi.org/10.
1039/C5AN02640G
Brady D, Jordaan J (2009) Advances in enzyme immobilisation. Biotechnol Lett 31
(11):1639–1650. https://doi.org/10.1007/s10529-009-0076-4
Cammarota MC, Freire DMG (2006) A review on hydrolytic enzymes in the treatment of wastewater with high oil and grease content. Bioresour Technol 97(17):2195–2210. https://doi.org/
10.1016/j.biortech.2006.02.030
Cancar HD, Soylemez S, Akpinar Y, Kesik M, Göker S, Gunbas G, Volkan M, Toppare L (2016) A
novel acetylcholinesterase biosensor: core–shell magnetic nanoparticles incorporating a conjugated polymer for the detection of organophosphorus pesticides. ACS Appl Mater Interfaces 8
(12):8058–8067. https://doi.org/10.1021/acsami.5b12383
Cao L (2006) Introduction: immobilized enzymes: past, present and prospects. In: Carrier-bound
immobilized enzymes. https://doi.org/10.1002/3527607668.ch1
Cao L, van Langen L, Sheldon RA (2003) Immobilised enzymes: carrier-bound or carrier-free?
Curr Opin Biotechnol 14(4):387–394. https://doi.org/10.1016/S0958-1669(03)00096-X
Cao S-L, Huang Y-M, Li X-H, Xu P, Wu H, Li N, Lou W-Y, Zong M-H (2016) Preparation and
characterization of immobilized lipase from pseudomonas cepacia onto magnetic cellulose
nanocrystals. Sci Rep 6(1):20420. https://doi.org/10.1038/srep20420
Chang Q, Tang H (2014) Immobilization of horseradish peroxidase on NH 2 -modified magnetic
Fe 3 O 4 /SiO 2 particles and its application in removal of 2,4-dichlorophenol. Molecules 19
(10):15768–15782. https://doi.org/10.3390/molecules191015768
Chang Q, Jiang G, Tang H, Li N, Huang J, Wu L (2015) Enzymatic removal of chlorophenols using
horseradish peroxidase immobilized on superparamagnetic Fe 3 O 4 /graphene oxide
nanocomposite. Chin J Catal 36(7):961–968. https://doi.org/10.1016/S1872-2067(15)60856-7
Chao C, Liu J, Wang J, Zhang Y, Zhang B, Zhang Y, Xiang X, Chen R (2013) Surface modification
of halloysite nanotubes with dopamine for enzyme immobilization. ACS Appl Mater Interfaces
5(21):10559–10564. https://doi.org/10.1021/am4022973
16 Enzyme-Based Nanomaterials in Bioremediation
365
review. Biotechnol Adv 30(3):512–523. https://doi.org/10.1016/j.biotechadv.2011.09.005
Arca-Ramos A, Ammann EM, Gasser CA, Nastold P, Eibes G, Feijoo G, Lema JM, Moreira MT,
Corvini PF-X (2016) Assessing the use of nanoimmobilized laccases to remove micropollutants
from wastewater. Environ Sci Pollut Res 23(4):3217–3228. https://doi.org/10.1007/s11356015-5564-6
Ardao I, Magnin D, Agathos SN (2015) Bioinspired production of magnetic laccase-biotitania
particles for the removal of endocrine disrupting chemicals. Biotechnol Bioeng 112
(10):1986–1996. https://doi.org/10.1002/bit.25612
Arora PK, Srivastava A, Singh VP (2010) Application of monooxygenases in dehalogenation,
desulphurization, denitrification and hydroxylation of aromatic compounds. J Bioremed
Biodegr 1(3):1000112. https://doi.org/10.4172/2155-6199.1000112
Ba S, Haroune L, Cruz-Morató C, Jacquet C, Touahar IE, Bellenger J-P, Legault CY, Jones JP,
Cabana H (2014) Synthesis and characterization of combined cross-linked laccase and tyrosinase aggregates transforming acetaminophen as a model phenolic compound in wastewaters.
Sci Total Environ 487(1):748–755. https://doi.org/10.1016/j.scitotenv.2013.10.004
Badgujar KC, Sasaki T, Bhanage BM (2015) Synthesis of lipase nano-bio-conjugates as an efficient
biocatalyst: characterization and activity–stability studies with potential biocatalytic applications. RSC Adv 5(68):55238–55251. https://doi.org/10.1039/C5RA10032A
Bansal N, Kanwar SS (2013) Peroxidase(s) in environment protection. Sci World J 2013:714639.
https://doi.org/10.1155/2013/714639
Bilal M, Iqbal HMN, Hu H, Wang W, Zhang X (2017) Development of horseradish peroxidasebased cross-linked enzyme aggregates and their environmental exploitation for bioremediation
purposes. J Environ Manag 188:137–143. https://doi.org/10.1016/j.jenvman.2016.12.015
Boujakhrout A, Jimenez-Falcao S, Martínez-Ruiz P, Sánchez A, Díez P, Pingarrón JM, Villalonga
R (2016) Novel reduced graphene oxide–glycol chitosan nanohybrid for the assembly of an
amperometric enzyme biosensor for phenols. Analyst 141(13):4162–4169. https://doi.org/10.
1039/C5AN02640G
Brady D, Jordaan J (2009) Advances in enzyme immobilisation. Biotechnol Lett 31
(11):1639–1650. https://doi.org/10.1007/s10529-009-0076-4
Cammarota MC, Freire DMG (2006) A review on hydrolytic enzymes in the treatment of wastewater with high oil and grease content. Bioresour Technol 97(17):2195–2210. https://doi.org/
10.1016/j.biortech.2006.02.030
Cancar HD, Soylemez S, Akpinar Y, Kesik M, Göker S, Gunbas G, Volkan M, Toppare L (2016) A
novel acetylcholinesterase biosensor: core–shell magnetic nanoparticles incorporating a conjugated polymer for the detection of organophosphorus pesticides. ACS Appl Mater Interfaces 8
(12):8058–8067. https://doi.org/10.1021/acsami.5b12383
Cao L (2006) Introduction: immobilized enzymes: past, present and prospects. In: Carrier-bound
immobilized enzymes. https://doi.org/10.1002/3527607668.ch1
Cao L, van Langen L, Sheldon RA (2003) Immobilised enzymes: carrier-bound or carrier-free?
Curr Opin Biotechnol 14(4):387–394. https://doi.org/10.1016/S0958-1669(03)00096-X
Cao S-L, Huang Y-M, Li X-H, Xu P, Wu H, Li N, Lou W-Y, Zong M-H (2016) Preparation and
characterization of immobilized lipase from pseudomonas cepacia onto magnetic cellulose
nanocrystals. Sci Rep 6(1):20420. https://doi.org/10.1038/srep20420
Chang Q, Tang H (2014) Immobilization of horseradish peroxidase on NH 2 -modified magnetic
Fe 3 O 4 /SiO 2 particles and its application in removal of 2,4-dichlorophenol. Molecules 19
(10):15768–15782. https://doi.org/10.3390/molecules191015768
Chang Q, Jiang G, Tang H, Li N, Huang J, Wu L (2015) Enzymatic removal of chlorophenols using
horseradish peroxidase immobilized on superparamagnetic Fe 3 O 4 /graphene oxide
nanocomposite. Chin J Catal 36(7):961–968. https://doi.org/10.1016/S1872-2067(15)60856-7
Chao C, Liu J, Wang J, Zhang Y, Zhang B, Zhang Y, Xiang X, Chen R (2013) Surface modification
of halloysite nanotubes with dopamine for enzyme immobilization. ACS Appl Mater Interfaces
5(21):10559–10564. https://doi.org/10.1021/am4022973
16 Enzyme-Based Nanomaterials in Bioremediation
365