General Note
Successful analyses of crude extracts and complex mixtures of analytes need a
fractionation step. This procedure is mainly done by low-performance liquid
column chromatography resulting in several subfractions containing different
substance classes with increasing polarity.
References
Cammann K (2010) Instrumentelle Analytische Chemie. Spektrum Akademischer, Heidelberg.
isbn:978-3-8274-2739-7
Schwedt G (2007) Taschenatlas der Analytik. Wiley-VCH, Weinheim. isbn:978-3-527-31729-5
Pentane
CH2Cl2/C5
60/40
MeOH/
DCM
1. Fract ion
AliphaƟcs
2. Fract ion
AromaƟcs
3. Fract ion
NSO-compounds
Pentane/CH2Cl2
90:10
1. Fract ion
Aliphat ics
2. Fract ion
Monoaromat ics
3. Fract ion
Diaromat ics
4. Fract ion
Polyaromat ics
5. Fract ion
Semipolars
6. Fract ion
Polars
Pentane Pentane/CH2Cl2
95:5
DCM
MeOH
HCB
PCN
Pentane/CH2Cl2
40:60
PCB
DDX
Carbamazepine
TMDD
Eluent
Eluent
Fig. 3.21 Typical fractionation scheme for fossil material (e.g. coals) and environmental extracts
(e.g. from sediment or soil samples, examples of corresponding pollutants are given)
References
37