Chapter 13
Some Frontier Technologies
for Aptamers in Medical Applications
Yiyang Dong, Sai Wang, Lianhui Zhao, Jinbin Yang, and Yan Xu
Abstract Up to now, many medical applications have been widely reported based
on the successful development of novel aptamers and continuous evolution of relevant aptamer-based technologies. However, class-specific aptamers are currently
not available, high-throughput aptamer affinity characterization tools are scarce,
and the stoichiometric info of aptamer-target complexes cannot be easily procured
for advanced structural biology or quantitative analytical chemistry research. In
this chapter, some cutting-edge technologies, as exemplified by UVMag-SELEX,
aptamer biochip, and aptamer oriented native mass spectrometry, are selected with
discretion from many aptamer-based frontier technologies and well presented for
further expanded medical applications.
Keywords Aptamers · UVMag-SELEX · Aptamer microarray biochip · Aptamer
microfluidic biochip · Nanoarray and nanofluidic biochip · Native mass
spectrometry
Y. Dong (B) · L. Zhao
College of Life Science and Technology, Beijing University of Chemical Technology, No.15
Beisanhuan East Road, Chaoyang District, Beijing 100029, People’s Republic of China
e-mail: yydong@mail.buct.edu.cn
S. Wang
College of Food Science and Engineering, Ocean University of China, Qingdao 266003, People’s
Republic of China
J. Yang · Y. Xu
Department of Chemical Engineering, Graduate School of Engineering, Osaka Prefecture
University, 1-2 Gakuen-Cho, Naka-Ku, Sakai, Osaka 599-8570, Japan
Y. Xu
Japan Science and Technology Agency (JST), PRESTO, 4-1-8 Honcho, Kawaguchi, Saitama
332-0012, Japan
NanoSquare Research Institute, Research Organization for the 21st Century, Osaka Prefecture
University, 1-2 Gakuen-Cho, Naka-Ku, Sakai, Osaka, Osaka 599-8570, Japan
© Springer Nature Singapore Pte Ltd. 2021
Y. Dong (ed.), Aptamers for Medical Applications,
https://doi.org/10.1007/978-981-33-4838-7_13
375
Some Frontier Technologies
for Aptamers in Medical Applications
Yiyang Dong, Sai Wang, Lianhui Zhao, Jinbin Yang, and Yan Xu
Abstract Up to now, many medical applications have been widely reported based
on the successful development of novel aptamers and continuous evolution of relevant aptamer-based technologies. However, class-specific aptamers are currently
not available, high-throughput aptamer affinity characterization tools are scarce,
and the stoichiometric info of aptamer-target complexes cannot be easily procured
for advanced structural biology or quantitative analytical chemistry research. In
this chapter, some cutting-edge technologies, as exemplified by UVMag-SELEX,
aptamer biochip, and aptamer oriented native mass spectrometry, are selected with
discretion from many aptamer-based frontier technologies and well presented for
further expanded medical applications.
Keywords Aptamers · UVMag-SELEX · Aptamer microarray biochip · Aptamer
microfluidic biochip · Nanoarray and nanofluidic biochip · Native mass
spectrometry
Y. Dong (B) · L. Zhao
College of Life Science and Technology, Beijing University of Chemical Technology, No.15
Beisanhuan East Road, Chaoyang District, Beijing 100029, People’s Republic of China
e-mail: yydong@mail.buct.edu.cn
S. Wang
College of Food Science and Engineering, Ocean University of China, Qingdao 266003, People’s
Republic of China
J. Yang · Y. Xu
Department of Chemical Engineering, Graduate School of Engineering, Osaka Prefecture
University, 1-2 Gakuen-Cho, Naka-Ku, Sakai, Osaka 599-8570, Japan
Y. Xu
Japan Science and Technology Agency (JST), PRESTO, 4-1-8 Honcho, Kawaguchi, Saitama
332-0012, Japan
NanoSquare Research Institute, Research Organization for the 21st Century, Osaka Prefecture
University, 1-2 Gakuen-Cho, Naka-Ku, Sakai, Osaka, Osaka 599-8570, Japan
© Springer Nature Singapore Pte Ltd. 2021
Y. Dong (ed.), Aptamers for Medical Applications,
https://doi.org/10.1007/978-981-33-4838-7_13
375