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Q. Lv et al.
Fig. 9.2 The principle of multiplex SOMAmer affinity assay. a SOMAmers and samples are mixed
and allowed to bind to form cognate or non-cognate SOMAmer-target protein complexes (b). c The
biotin-modified SOMAmers-target complexes could be separated by streptavidin-labeled beads.
d The separated proteins are tagged with NHS-biotin for further purification. e The complexes are
released from the surface of the beads through photocleavage, and the non-cognate complexes dissociate preferentially. f The released complexes are re-captured through the biotin tag on protein with
streptavidin-modified beads. Finally, the SOMAmers are eluted at high pH (g) and the remaining
SOMAmers are quantified by DNA microarray technique (h). Reprinted with permission from Ref.
[28].
Q. Lv et al.
Fig. 9.2 The principle of multiplex SOMAmer affinity assay. a SOMAmers and samples are mixed
and allowed to bind to form cognate or non-cognate SOMAmer-target protein complexes (b). c The
biotin-modified SOMAmers-target complexes could be separated by streptavidin-labeled beads.
d The separated proteins are tagged with NHS-biotin for further purification. e The complexes are
released from the surface of the beads through photocleavage, and the non-cognate complexes dissociate preferentially. f The released complexes are re-captured through the biotin tag on protein with
streptavidin-modified beads. Finally, the SOMAmers are eluted at high pH (g) and the remaining
SOMAmers are quantified by DNA microarray technique (h). Reprinted with permission from Ref.
[28].