102
Z. Liu and Y. Liu
Besides DNA framework nanostructure, Holliday junction DNA device provides
another platform for targeted drug delivery. In one illustrative example, Tung et al.
[122] developed a novel Holliday junction DNA device where three arms carried
three siRNA strands (AKT, MDM2, and Survivin) as therapeutic agents, and the
remaining arms were linked to AS1411 aptamer for cell targeting (Fig. 4.9a). The
platform with high selectivity maintained its morphological characteristics during
cellular uptake and exhibited 80% inhibition on breast MDA-MB-231 cancer cells.
In a recent study, Yao et al. [123] developed a novel selective drug delivery system
(SDDS) containing self-assembled DNA nanocross (Holliday junction, or HJ) modified with four AS1411 aptamers, to deliver DOX to colon cancer cells. As shown in
Fig. 4.9b, four DNA sequences assembled automatically and formed nanocross based
on complementary base pairing. DOX was intercalated into the G–C pairs. Each DNA
Fig. 4.9 a The internalization of Holliday junction DNA devices bearing siRNA and aptamers into
cells and subsequent cellular disassembly. Reprinted with permission from Ref. [122] Copyright
2017, Springer Nature. b Self-assembled DNA nano-cross (Holliday junction, or HJ) modified by
four AS1411 aptamers. Reprinted with permission from Ref. [123] Copyright 2020. Dove Medical
Press Limited. c DNA nanostructure with various architectures assembled from DNA strands.
Reprinted with permission from Ref. [111] Copyright 2020, John Wiley & Sons Ltd. d Structureswitching aptamer which can induce hybridization chain reaction on the cell surface for activatable theranostic. Reprinted with permission from Ref. [127] Copyright 2015, American Chemical
Society. e pH-stimulated multivalent DNA assemblies (MDAs). Reprinted with permission from
Ref. [128] Copyright 2020, The Royal Society of Chemistry. f D-PGM for three-receptor-mediated
cell identification and targeted cancer therapy. Reprinted with permission from Ref. [129] Copyright
2020, American Chemical Society. g DNA framework for logic imaging of lysosomal pH and ATP.
Reprinted with permission from Ref. [131] Copyright 2019, American Chemical Society
Z. Liu and Y. Liu
Besides DNA framework nanostructure, Holliday junction DNA device provides
another platform for targeted drug delivery. In one illustrative example, Tung et al.
[122] developed a novel Holliday junction DNA device where three arms carried
three siRNA strands (AKT, MDM2, and Survivin) as therapeutic agents, and the
remaining arms were linked to AS1411 aptamer for cell targeting (Fig. 4.9a). The
platform with high selectivity maintained its morphological characteristics during
cellular uptake and exhibited 80% inhibition on breast MDA-MB-231 cancer cells.
In a recent study, Yao et al. [123] developed a novel selective drug delivery system
(SDDS) containing self-assembled DNA nanocross (Holliday junction, or HJ) modified with four AS1411 aptamers, to deliver DOX to colon cancer cells. As shown in
Fig. 4.9b, four DNA sequences assembled automatically and formed nanocross based
on complementary base pairing. DOX was intercalated into the G–C pairs. Each DNA
Fig. 4.9 a The internalization of Holliday junction DNA devices bearing siRNA and aptamers into
cells and subsequent cellular disassembly. Reprinted with permission from Ref. [122] Copyright
2017, Springer Nature. b Self-assembled DNA nano-cross (Holliday junction, or HJ) modified by
four AS1411 aptamers. Reprinted with permission from Ref. [123] Copyright 2020. Dove Medical
Press Limited. c DNA nanostructure with various architectures assembled from DNA strands.
Reprinted with permission from Ref. [111] Copyright 2020, John Wiley & Sons Ltd. d Structureswitching aptamer which can induce hybridization chain reaction on the cell surface for activatable theranostic. Reprinted with permission from Ref. [127] Copyright 2015, American Chemical
Society. e pH-stimulated multivalent DNA assemblies (MDAs). Reprinted with permission from
Ref. [128] Copyright 2020, The Royal Society of Chemistry. f D-PGM for three-receptor-mediated
cell identification and targeted cancer therapy. Reprinted with permission from Ref. [129] Copyright
2020, American Chemical Society. g DNA framework for logic imaging of lysosomal pH and ATP.
Reprinted with permission from Ref. [131] Copyright 2019, American Chemical Society