Table 1
Main fluorogen/RNA light-up aptamer pairs and their properties
Fluorogen
Light-up
aptamer
Aptamer selection
strategy
K D (nM) Ex./Em. (nm)
Abs. Coef.
(ε)
Q.Y.
Brightness
Rel.
Brightness Reference
eGFP
Natural molecule /
490/508
39,200
0.68 26.60
1.00
[36]
OTB-SO3
DiR2s
SELEX
662
380/421
73,000
0.51 37.23
1.40
[17]
Hoescht-1c Apt II-mini3-4 SELEX
35
345/470
n.a
0.26 n.a
n.a.
[12, 13]
DFHBI
Spinach
SELEX
540
469/501
24,300
0.72 17.50
0.65
[18]
DFHBI
iSpinach
μIVC
920
442/503
n.a.
n.a.
n.a.
n.a.
[42]
DFHBI-1T
Spinach2
SELEX/design
560
482/505
31,000
0.94 29.10
1.10
[19]
DFHBI-1T
Broccoli
SELEX/FACS
360
472/507
29,600
0.94 27.80
1.04
[35]
TO1-Biotin iMangoIII
μIVC
4
506/527
77,500
0.64 49.6
1.86
[55]
RG-DN
DNB
SELEX
4480
507/534
37,350
0.32 11.90
0.44
[28]
TO1-Biotin Mango
SELEX
3
510/535
77,500
0.14 10.85
0.40
[14]
TO1-Biotin MangoII
μIVC
1
510/535
77,500
0.21 16.28
0.61
[43]
TO1-Biotin MangoIII
μIVC
5
510/534
77,500
0.55 42.63
1.6
[43]
DMHBI+
Chili
SELEX/design
63
413/542
n.a.
0.40 n.a.
n.a.
[22]
DMHBI-Imi Chili
SELEX/design
71
463/545, 594 n.a.
0.08 n.a.
n.a.
[22]
DFHO
Corn
SELEX
70
505/545
29,000
0.25 7.25
0.27
[21]
DFHO
Orange Broccoli SELEX
230
513/562
34,000
0.28 9.52
0.36
[21]
CY3-BHQ1 BHQ apt (A1)
SELEX
n.a.
520/565
n.a.
n.a.
n.a.
n.a.
[31]
DFHO
Red-Broccoli
SELEX
206
518/582
35,000
0.34 11.90
0.44
[21]
TMR-DN
SRB2
SELEX
35
564/587
90,500
0.33 29.87
1.12
[29]
SR-DN
DNB
SELEX
800
572/591
50,250
0.98 49.24
1.85
[28]
DMHBO+
Chili
SELEX/design
12
456/592
n.a.
0.10 n.a.
n.a.
[22]
SR-DN
SRB2
SELEX
1340
579/596
85,200
0.65 55.38
2.08
[29]
Cbl-Atto590 Riboglow
Natural molecule 3-34
594/624
120,000 0.31 37.2
1.40
[32]
DIR
DIR apt
SELEX
86
600/646
134,000 0.26 34.80
1.30
[16]
Mal. Green
MGA
SELEX
117
630/650
150,000 0.19 28.00
1.05
[7, 11]
DIR-pro
DIR2s-Apt
SELEX
252
600/658
164,000 0.33 54.12
2.00
[17]
TO3-Biotin Mango
SELEX
6-8
637/658
9,300
n.a.
n.a.
n.a.
[14]
SiR-DN
SiRA
SELEX
1000
650/662
n.a.
n.a.
n.a.
n.a.
[30]
Cbl-Cy5
Riboglow
Natural molecule n.a.
646/662
271,000 0.26 70.46
2.65
[32]
Patent Blue SRB apt
SELEX
23
n.a./665
n.a.
0.034 n.a.
n.a.
[7]
SiR-linker
SiRA
SELEX
430
649/666
86,000
0.98 84.28
3.16
[30]
76
Michael Ryckelynck
Main fluorogen/RNA light-up aptamer pairs and their properties
Fluorogen
Light-up
aptamer
Aptamer selection
strategy
K D (nM) Ex./Em. (nm)
Abs. Coef.
(ε)
Q.Y.
Brightness
Rel.
Brightness Reference
eGFP
Natural molecule /
490/508
39,200
0.68 26.60
1.00
[36]
OTB-SO3
DiR2s
SELEX
662
380/421
73,000
0.51 37.23
1.40
[17]
Hoescht-1c Apt II-mini3-4 SELEX
35
345/470
n.a
0.26 n.a
n.a.
[12, 13]
DFHBI
Spinach
SELEX
540
469/501
24,300
0.72 17.50
0.65
[18]
DFHBI
iSpinach
μIVC
920
442/503
n.a.
n.a.
n.a.
n.a.
[42]
DFHBI-1T
Spinach2
SELEX/design
560
482/505
31,000
0.94 29.10
1.10
[19]
DFHBI-1T
Broccoli
SELEX/FACS
360
472/507
29,600
0.94 27.80
1.04
[35]
TO1-Biotin iMangoIII
μIVC
4
506/527
77,500
0.64 49.6
1.86
[55]
RG-DN
DNB
SELEX
4480
507/534
37,350
0.32 11.90
0.44
[28]
TO1-Biotin Mango
SELEX
3
510/535
77,500
0.14 10.85
0.40
[14]
TO1-Biotin MangoII
μIVC
1
510/535
77,500
0.21 16.28
0.61
[43]
TO1-Biotin MangoIII
μIVC
5
510/534
77,500
0.55 42.63
1.6
[43]
DMHBI+
Chili
SELEX/design
63
413/542
n.a.
0.40 n.a.
n.a.
[22]
DMHBI-Imi Chili
SELEX/design
71
463/545, 594 n.a.
0.08 n.a.
n.a.
[22]
DFHO
Corn
SELEX
70
505/545
29,000
0.25 7.25
0.27
[21]
DFHO
Orange Broccoli SELEX
230
513/562
34,000
0.28 9.52
0.36
[21]
CY3-BHQ1 BHQ apt (A1)
SELEX
n.a.
520/565
n.a.
n.a.
n.a.
n.a.
[31]
DFHO
Red-Broccoli
SELEX
206
518/582
35,000
0.34 11.90
0.44
[21]
TMR-DN
SRB2
SELEX
35
564/587
90,500
0.33 29.87
1.12
[29]
SR-DN
DNB
SELEX
800
572/591
50,250
0.98 49.24
1.85
[28]
DMHBO+
Chili
SELEX/design
12
456/592
n.a.
0.10 n.a.
n.a.
[22]
SR-DN
SRB2
SELEX
1340
579/596
85,200
0.65 55.38
2.08
[29]
Cbl-Atto590 Riboglow
Natural molecule 3-34
594/624
120,000 0.31 37.2
1.40
[32]
DIR
DIR apt
SELEX
86
600/646
134,000 0.26 34.80
1.30
[16]
Mal. Green
MGA
SELEX
117
630/650
150,000 0.19 28.00
1.05
[7, 11]
DIR-pro
DIR2s-Apt
SELEX
252
600/658
164,000 0.33 54.12
2.00
[17]
TO3-Biotin Mango
SELEX
6-8
637/658
9,300
n.a.
n.a.
n.a.
[14]
SiR-DN
SiRA
SELEX
1000
650/662
n.a.
n.a.
n.a.
n.a.
[30]
Cbl-Cy5
Riboglow
Natural molecule n.a.
646/662
271,000 0.26 70.46
2.65
[32]
Patent Blue SRB apt
SELEX
23
n.a./665
n.a.
0.034 n.a.
n.a.
[7]
SiR-linker
SiRA
SELEX
430
649/666
86,000
0.98 84.28
3.16
[30]
76
Michael Ryckelynck