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repression. Nat Commun 7:13031
12. Huang K, Baldrich P, Meyers BC, Caplan JL
(2019) sRNA-FISH: versatile fluorescent in
situ detection of small RNAs in plants. Plant J
98:359–369. https://doi.org/10.1111/tpj.
14210
13. Schindelin J, Arganda-Carreras I, Frise E,
Kaynig V, Longair M, Pietzsch T, Preibisch S,
Rueden C, Saalfeld S, Schmid B, Tinevez JY,
White DJ, Hartenstein V, Eliceiri K,
Tomancak P, Cardona A (2012) Fiji: an opensource platform for biological-image analysis.
Nat Methods 9(7):676–682. https://doi.org/
10.1038/Nmeth.2019
14. Mueller F, Senecal A, Tantale K, Marie-NellyH, Ly N, Collin O, Basyuk E, Bertrand E,
Darzacq X, Zimmer C (2013) FISH-quant:
automatic counting of transcripts in 3D FISH
images. Nat Methods 10(4):277–278
Single-Molecule FISH Imaging of RNAs in Plants
33
