Cas9. Methods Mol Biol 1311:335–348.
https://doi.org/10.1007/978-1-4939-26879_22
16. Kondo S, Ueda R (2013) Highly improved
gene targeting by germline-specific Cas9
expression in Drosophila. Genetics 195
(3):715–721.
https://doi.org/10.1534/
genetics.113.156737
17. Bischof J, Maeda RK, Hediger M, Karch F,
Basler K (2007) An optimized transgenesis system for Drosophila using germ-line-specific
phiC31 integrases. Proc Natl Acad Sci U S A
104(9):3312–3317.
https://doi.org/10.
1073/pnas.0611511104
18. Chen H, Levo M, Barinov L, Fujioka M, Jaynes
JB, Gregor T (2018) Dynamic interplay
between enhancer-promoter topology and
gene activity. Nat Genet 50(9):1296–1303.
https://doi.org/10.1038/s41588-018-0175-z
19. Chen H, Xu Z, Mei C, Yu D, Small S (2012) A
system of repressor gradients spatially organizes the boundaries of Bicoid-dependent target genes. Cell 149(3):618–629. https://doi.
org/10.1016/j.cell.2012.03.018
20. Bateman JR, Lee AM, Wu CT (2006) Sitespecific transformation of Drosophila via
phiC31 integrase-mediated cassette exchange.
Genetics 173(2):769–777. https://doi.org/
10.1534/genetics.106.056945
21. Garcia HG, Gregor T (2018) Live imaging of
mRNA synthesis in Drosophila. Methods Mol
Biol 1649:349–357. https://doi.org/10.
1007/978-1-4939-7213-5_23
22. Gratz SJ, Cummings AM, Nguyen JN, Hamm
DC,
Donohue
LK,
Harrison
MM,
Wildonger J, O’Connor-Giles KM (2013)
Genome engineering of Drosophila with the
CRISPR RNA-guided Cas9 nuclease. Genetics
194(4):1029–1035.
https://doi.org/10.
1534/genetics.113.152710
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