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dual-color-labeled CRISPR system. Anal
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org/10.1021/acs.analchem.7b03584
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hundred
kilobases.
Nat
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(5):343–345.
https://doi.org/10.1038/
nmeth.1318
31. Schindelin J, Arganda-Carreras I, Frise E,
Kaynig V, Longair M, Pietzsch T, Preibisch S,
Rueden C, Saalfeld S, Schmid B, Tinevez JY,
White DJ, Hartenstein V, Eliceiri K,
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org/10.1016/j.biomaterials.2016.05.022
372
Xiaotian Wu et al.
https://doi.org/10.1126/science.aao6135
22. Cheng AW, Jillette N, Lee P, Plaskon D,
Fujiwara Y, Wang W, Taghbalout A, Wang H
(2016) Casilio: a versatile CRISPR-Cas9Pumilio hybrid for gene regulation and genomic labeling. Cell Res 26(2):254–257. https://
doi.org/10.1038/cr.2016.3
23. Ma H, Tu LC, Naseri A, Chung YC,
Grunwald D, Zhang S, Pederson T (2018)
CRISPR-Sirius: RNA scaffolds for signal
amplification in genome imaging. Nat Methods 15(11):928–931. https://doi.org/10.
1038/s41592-018-0174-0
24. Ma H, Tu LC, Naseri A, Huisman M, Zhang S,
Grunwald D, Pederson T (2016) CRISPRCas9 nuclear dynamics and target recognition
in living cells. J Cell Biol 214(5):529–537.
https://doi.org/10.1083/jcb.201604115
25. Deng W, Shi X, Tjian R, Lionnet T, Singer RH
(2015) CASFISH: CRISPR/Cas9-mediated in
situ labeling of genomic loci in fixed cells. Proc
Natl Acad Sci U S A 112(38):11870–11875.
https://doi.org/10.1073/pnas.1515692112
26. Knight SC, Xie L, Deng W, Guglielmi B, Witkowsky LB, Bosanac L, Zhang ET, El
Beheiry M, Masson JB, Dahan M, Liu Z,
Doudna JA, Tjian R (2015) Dynamics of
CRISPR-Cas9 genome interrogation in living
cells. Science 350(6262):823–826. https://
doi.org/10.1126/science.aac6572
27. Wu X, Mao S, Yang Y, Rushdi MN, Krueger
CJ, Chen AK (2018) A CRISPR/molecular
beacon hybrid system for live-cell genomic
imaging. Nucleic Acids Res 46(13):e80.
https://doi.org/10.1093/nar/gky304
28. Ma Y, Wang M, Li W, Zhang Z, Zhang X,
Wu G, Tan T, Cui Z, Zhang XE (2017) Live
visualization of HIV-1 proviral DNA using a
dual-color-labeled CRISPR system. Anal
Chem 89(23):12896–12901. https://doi.
org/10.1021/acs.analchem.7b03584
29. Tyagi S, Kramer FR (1996) Molecular beacons:
probes that fluoresce upon hybridization. Nat
Biotechnol 14(3):303–308. https://doi.org/
10.1038/nbt0396-303
30. Gibson DG, Young L, Chuang RY, Venter JC,
Hutchison CA 3rd, Smith HO (2009) Enzymatic assembly of DNA molecules up to several
hundred
kilobases.
Nat
Methods
6
(5):343–345.
https://doi.org/10.1038/
nmeth.1318
31. Schindelin J, Arganda-Carreras I, Frise E,
Kaynig V, Longair M, Pietzsch T, Preibisch S,
Rueden C, Saalfeld S, Schmid B, Tinevez JY,
White DJ, Hartenstein V, Eliceiri K,
Tomancak P, Cardona A (2012) Fiji: an opensource platform for biological-image analysis.
Nat Methods 9(7):676–682. https://doi.org/
10.1038/nmeth.2019
32. Zhao D, Yang Y, Qu N, Chen M, Ma Z, Krueger CJ, Behlke MA, Chen AK (2016) Singlemolecule detection and tracking of RNA transcripts in living cells using phosphorothioateoptimized 2’-O-methyl RNA molecular beacons. Biomaterials 100:172–183. https://doi.
org/10.1016/j.biomaterials.2016.05.022
372
Xiaotian Wu et al.